毛细管气相色谱内标法同时测定藏木香中土木香内酯、异土木香内酯的含量  

GC determination of alantolactone and isoalantolactone in Zangmuxiang

作　　者：王燕[1] 张雅惠[1] 格桑索朗 孙文基[1] 

机构地区：[1]西北大学陕西省生物医药重点实验室,西安710069 [2]西藏药品检验所,850000

出　　处：《药物分析杂志》2009年第12期2058-2060,共3页Chinese Journal of Pharmaceutical Analysis

基　　金：中国药典2010年版研究项目(国家药典中发[2008]99号)

摘　　要：目的:测定藏木香药材中土木香内酯、异土木香内酯的含量。方法:用SE-30毛细管柱(30m×0.32mm×0.25μm);检测器温度为260℃(FID);进样口温度240℃,程序升温:初始温度50℃,以20℃·min-1的速率升温至170℃,保持28min,再以25℃·min-1的速率升温至250℃,保持1min;以尼泊金丁酯为内标物定量。结果:土木香内酯与异土木香内酯分离良好,在0.6～4.2mg·mL-1范围内,呈良好的线性关系(r=0.9999)。结论:本法简便、灵敏,专属性强,重复性好,可用于藏木香药材的含量测定,控制内在质量。Objective：A capillary GC method was established for the determination of alantolactone and isoalantolactone in Zangmuxiang.Method：The analytical column was SE-30 capillary column（30 m×0.32 mm×0.25 μm）with an FID detector.The injector temperature was 240 ℃.The detector temperature was set at 260 ℃.The column temperature was programmed from 50 ℃ at a rate of 20 ℃·min-1 to 170 ℃（holding 28 min）,then raised to 250 ℃ at 25 ℃·min-1.The final temperature was maintained for 1 min.Results：The linear ranges of alantolactone and isoalantolactone were 0.6-4.2 mg·mL-1 （r=0.9999）,which showed good linearity.Conclution：The methed is simple,specificity,accurate and reliable,and can be used for quality control of Zangmuxiang.

关 键 词：气相色谱法 藏木香 土木香内酯 异土木香内酯 

分 类 号：R917[医药卫生—药物分析学]