咖啡酸乙酯与DNA相互作用的电化学及紫外光谱研究  

Electrochemical and UV spectroscopic studies on the interaction between ethyl caffeate and DNA

作　　者：于婷婷[1] 何平[1] 梁艳[1] 蒋琪英[1] 

机构地区：[1]西南科技大学材料科学与工程学院,绵阳621010

出　　处：《药物分析杂志》2009年第12期2088-2092,共5页Chinese Journal of Pharmaceutical Analysis

基　　金：四川省教育厅自然科学基金项目(06ZD1105)资助

摘　　要：目的:研究咖啡酸乙酯与DNA的相互作用。方法:采用循环伏安法和差分脉冲伏安法并结合紫外吸收光谱法研究了咖啡酸乙酯与鲱鱼精DNA(HS-DNA)的相互作用。结果:在0.1mol.L-1磷酸盐缓冲溶液(pH7.4)中,在玻碳电极上咖啡酸乙酯的循环伏安曲线上有一对氧化还原峰,其峰电流随着HS-DNA的加入而下降,式量电位负移。结论:咖啡酸乙酯与HS-DNA的相互作用以静电方式为主,通过测定HS-DNA引入前后的电化学参数,推测咖啡酸乙酯与HS-DNA在该条件下结合生成了一种非电活性的超分子化合物,其结合比和结合常数分别为2和9.8×106。紫外吸收光谱进一步验证了以上这些结论。Objective：To investigate the interaction between ethyl caffeate and herring sperm DNA （HS-DNA）.Methods：The interaction between ethyl caffeate and herring sperm DNA was investigated by cyclic voltammetry,differential pulse voltammetry and ultraviolet absorption spectrometry.Results：It was shown that ethyl caffeate exhibited a pair of electrochemical redox peaks in 0.1 mol·L-1 phosphate buffer （PBS,pH 7.4） at glassy carbon electrode.Meanwhile,both the cathode and anode peak currents decreased with increasing the amount of HS-DNA,and the formal potential shifted negatively.Conclusions：The interaction between ethyl caffeate and HS-DNA was mainly of an electrostatic one in phosphate buffer （pH 7.4）,by the determination of electrochemical parameters in the absence and the presence of HS-DNA,it was deduced that a non-electroactive supramolecular compound was formed between ethyl caffeate and HS-DNA.The molar binding ratio and the binding constant of ethyl ferulate into HS-DNA were calculated to be 2 and 9.8×106,respectively.Ultraviolet absorption spectroscopy has validated the above conclusion.

关 键 词：伏安法 紫外吸收光谱 咖啡酸乙酯 鲱鱼精DNA 

分 类 号：R917[医药卫生—药物分析学]