HPLC-ELSD测定知母中知母皂苷BⅡ的含量  

HPLC-ELSD determination of timosaponin BⅡ in Anemarrhena asphodeloides Bge.

作　　者：沙东旭[1] 刘兆妍[1] 张满来[1] 宋少江[2] 

机构地区：[1]辽宁省食品药品检验所,沈阳110023 [2]沈阳药科大学,沈阳110016

出　　处：《药物分析杂志》2009年第12期2106-2108,共3页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:采用HPLC-ELSD法测定知母中知母皂苷BⅡ的含量,为质量标准的建立提供数据。方法:采用Agilent TC-C18(250mm×4.6mm,5μm)色谱柱,以乙腈-水(25∶75)为流动相,流速0.8mL·min-1,漂移管温度为105℃,载气流速为3.0L·min-1。结果:知母皂苷BⅡ进样量在0.785～19.6μg范围内呈良好线性关系;在药材中的平均回收率(n=6)为103.1%,RSD为1.0%。不同来源知母药材和饮片(各10批)中知母皂苷BⅡ的含量在3.84%～9.91%范围内。结论:本方法简便、准确,无干扰,可用于知母中知母皂苷BⅡ的含量测定。Objective：To establish the quantitative method for timosaponin BⅡ in Anemarrhena asphodeloides Bge.by HPLC-ELSD.Method：The column used was a packed with 5 μm stationary phase Agilent TC-C18（250 mm×4.6 mm）.The mobile phase consisted of acetonitrile-water（25∶75） at a flow rate of 0.8 mL·min-1;The temperature of drift tube was 105 ℃,and the nebulizer nitrogen flow rate was 3.0 L·min-1.Result：The linear range of the component was 0.785-19.6 μg;The average recovery（n=6） of timosaponin BⅡ in crude material was 103.1% with RSD of 1.0%.The contents of timosaponin BⅡ in crude form and slices of Anemarrhena asphodeloides from different sourses were 3.84%-9.91%.Conclusion：The method is reliable,simple and precise,and it can be used for the determination of Anemarrhena asphodeloides Bge.

关 键 词：知母 知母皂苷BⅡ HPLC-ELSD 

分 类 号：R917[医药卫生—药物分析学]