栽培大豆种质资源对大豆菌核病的抗性评价  

Identification of Resistance to Sclerotinia Sclerotiorum in Soybean Germplasm

作　　者：王金生[1] 于安亮[1] 徐鹏飞[1] 王君[2] 陈晨[1] 李宁辉[1] 李文滨[1] 王继安[1] 张淑珍[1] 

机构地区：[1]东北农业大学大豆研究所,教育部大豆生物学重点实验室,黑龙江哈尔滨150030 [2]东北农业大学农学院,黑龙江哈尔滨150030

出　　处：《大豆科学》2009年第6期1054-1057,共4页Soybean Science

基　　金：国家自然科学基金资助项目(30671317,30810103063,30400285);黑龙江省自然科学基金资助项目(C200814);黑龙江省新世纪人才培养计划资助项目(NCET-06-007);黑龙江省农业科学院博士后基金资助项目(LRB06-010);博士后落户黑龙江科研启动基金资助项目

摘　　要：采用离体叶柄接种法鉴定了200份栽培大豆高世代品系对大豆菌核病菌株Jia30和Jian29的抗感反应。结果表明:在所有的参鉴材料中没有发现免疫类型,但各品系间抗性有一定的差异。供试200个品系中既表现抗Jia30菌株又表现抗Jian29菌株的材料占供试材料的2%;抗Jia30菌株的材料占3%;抗Jian29菌株的材料占5%,根据抗性资源筛选结果,这些抗性材料可合理地用于大豆生产,并为大豆抗病育种亲本选择和利用品种布局进行大豆菌核病生态控制提供了依据。Sclerotinia stem rot of soybean,commonly called white mould,is a fungal disease caused by Sclerotinia sclerotiorum （Lib.）de Bery in the world. The disease distributed all throughout China, especially in Heilongjiang and Inner Mongolia. Heilongjiang is the nlain soybean production region in China,and white mould occurred seriously in recent years for the increase planting area of soybean, rape and sunflower. Utilization of resistant cuhivars is the most economic, efficient, and safe method to control this disease. Screening on the resistance germplasm is the basis for resistance breeding. In this study, the reaction of 200 soybean lines to Sclerotinia sclerotiorum isolates Jia30 and Jian29 were evaluated by leafstalk inoculation in- vitro under controlled conditions. The results showed that there was no immunity accssion among all the soybean lines tested, but there was difference in resistance among them. Four lines were resistant to the both isolates,representing 2% of all the accessions tested;Six lines were resistant to isolates Jia30, representing 3% of all the accessions tested;Ten lines were resistant to isolates Jian29,representing 5% of all the accessions tested. These lines could be used in soybean resistance breeding,and provide references for parent choosing in soybean resistant breeding and ecological control by arrangement of cultivars with different resistance.

关 键 词：大豆 菌核病 抗性鉴定 

分 类 号：S565.1[农业科学—作物学]