电针不同介入时间对急性脊髓损伤大鼠一氧化氮、超氧化物歧化酶和丙二醛的影响  

Influence of Electroacupuncture at Different Time on Nitric Oxide,Superoxide Dismutase and Malondialdehyde Levels in Acute Injury of Spinal Cord in Rats

作　　者：王婷婷[1] 邬继红[1] 钟煜朝[1] 韩丽[1] 王自勤[1] 

机构地区：[1]北京中医药大学针灸学院

出　　处：《中医杂志》2009年第12期1100-1102,共3页Journal of Traditional Chinese Medicine

基　　金：北京中医药大学校级课题

摘　　要：目的对电针治疗急性脊髓损伤(ASCI)大鼠的最佳介入时间进行初步探讨。方法将50只雄性SD大鼠随机分为空白组、模型组、电针组、电针组、电针组,电针各组分别在造模后2h、6h、12h进行电针治疗。选用"大椎"和"命门"穴,持续脉冲电流,频率2Hz,强度2～5mA,治疗30min。在造模后24h采集标本,检测一氧化氮(NO)、超氧化物歧化酶(SOD)和丙二醛(MDA)含量的变化。结果电针各组大鼠损伤脊髓中NO、SOD和MDA含量均较模型组有改变,以造模后2h针刺影响最明显(P<0.05或P<0.01),造模后6h针刺次之,造模后12h针刺再次之。结论急性脊髓损伤后早期介入(2h针刺)疗效更佳。Objective To observe the effect of electroacupuncture at different time in acute injury of spinal cord （ASCI） in rats so as to find the best time for the treatment. Methods A total of 50 SD rats were evenly randomized into normal group, model group, electroacupuncture group A,B and C. The 3 eleetroacupuncture groups were treated with electroacupuncture at 2h, 6h and 12h respectively after being made ASCI model. The acupoints Mingmen （GV 4） and Dazhui （GV 14） were chosen for electroacupuncture with frequency 2Hz, continuous wave, and intensity 2-5mA for 30minutes. The samples were collected at 24h after modeling to detect the changes in nitric oxide （NO）, malondialdehyde （MDA） and superoxide dismutase （SOD） in the injured spinal cord. Results After treatment, the serum levels of NO, MDA and SOD in the injured spinal cord of all rats changed. The result of 2h after modeling was the most significant （P〈0. 05 or P〈0. 01）, then 6h the second, and then 12h the third. Conclusion Electroacupuneture given earlier （2h after ASCI） achieves better effect.

关 键 词：急性脊髓损伤 一氧化氮 丙二醛 超氧化物歧化酶 针刺时效 

分 类 号：R245[医药卫生—针灸推拿学]