染色体脆性位点抑癌基因FHIT真核表达质粒的构建  

Construction of eukaryotic expression vector of tumor suppressor gene at Chromosome fragile sites FHIT

作　　者：谢放[1] 黄强[1] 

机构地区：[1]安徽医科大学附属省立医院,合肥230001

出　　处：《山东医药》2009年第42期21-22,共2页Shandong Medical Journal

摘　　要：目的构建人染色体脆性位点抑癌基因FHIT真核表达质粒。方法以通过全基因合成的FHIT cDNA为模版,用PCR技术扩增,扩增片段用BamHI/Xho I双酶切后克隆到真核表达载体pcDNA3.1中,用DNA测序法鉴定重组质粒。结果质粒DNA测序显示与文献报道的人FHIT cDNA序列一致。结论成功构建出含人FHIT基因的真核表达质粒。Objective To construct the eukaryotic expression vector of human tumor suppressor gene at chromosome fragile sites FHIT.Methods FHIT cDNA was whole-genome synthesized as a template for PCR.The fragment above was amplified with BamH I / Xho I double digestion and cloned into the eukaryotic expression vector pcDNA 3.1.The recombinant vector was identified by DNA sequencing.Result The sequence of target gene of recombinant vector pcDNA3.1-FHIT constructed in this experiment was in accordance with the sequence of human FHIT cDNA reported.Conclusion The eukaryotic expression vector pcDNA 3.1-FHIT has been constructed successfully.

关 键 词：抑癌基因 FHIT基因 真核表达载体 

分 类 号：R331[医药卫生—人体生理学]