黑胸散白蚁(Reticulitermes chinensis Snyder)肠道共生古菌的系统发育分析  

Phylogenetic analysis of symbiotic archaea in the gut of Reticulitermes chinensis Snyder

作　　者：石玉[1] 张燕鸿[1] 杨红[1,2] 

机构地区：[1]华中师范大学生命科学学院,遗传调控与整合生物学湖北省重点实验室,武汉430079 [2]华中师范大学昆虫所,农药与化学生物学教育部重点实验室,武汉430079

出　　处：《微生物学报》2009年第12期1655-1659,共5页Acta Microbiologica Sinica

基　　金：国家自然科学基金(30570034)~~

摘　　要：【目的】利用非培养法对黑胸散白蚁（Reticulitermes chinensis Snyder）肠道共生古菌进行系统发育分析。【方法】采用古菌16SrDNA通用引物以黑胸散白蚁全肠DNA为模板扩增共生菌的16SrDNA并建立基因文库,对得到的基因序列进行系统发育分析。【结果】从黑胸散白蚁肠道得到5个不同的16SrDNA序列,它们之间的相似性为93.2%-99.2%,系统发育分析表明这5个16SrDNA序列代表的克隆分别与来源于黑胸散白蚁近缘种,栖北散白蚁和北美散白蚁肠道中的甲烷短杆菌克隆或分离株的亲缘关系最近。【结论】黑胸散白蚁肠道共生古菌为甲烷短杆菌。[ Objective] To analyze the phylotypes of symbiotic archaea in the gut of Reticulitermes chinensis Snyder by using noncultivating method. [Methods] We amplified 16S rRNA genes of the symbiotic archaea from the DNA extract of the whole gut with the universal arcbaeal 16S rDNA primer. A clone library of archaeal 16S rDNA was established and selected clones were sequenced and phylogeneticly analyzed. [ Results] Five 16S rDNA sequences with the similarity between 93.2% and 99.2% to each other were obtained from Reticulitermes chinensis Snyder. Phylogenetic analysis showed that the five clones were clustered with Methanobrevibacter clones or isolates obtained from Reticulitermes speratus and Reticulitermes flavipes. [Conclusionl The results showed that symbiotic archaea in the gut of Reticulitermes chinensis were affiliated with Methanobrevibacter.

关 键 词：黑胸散白蚁 肠道 共生古菌 系统发育分析 

分 类 号：Q93[生物学—微生物学]