放射诱导基因Gadd45在健康人群中表达水平的检测及其意义  

Expression of irradiation inducible gene Gadd 45 in population

作　　者：徐小洁[1] 王凌雪[1] 蔺静[2] 丁丽华[1] 王荣福[3] 秦玺[1] 程龙[1] 康磊[3] 叶棋浓[1] 

机构地区：[1]军事医学科学院生物工程所,北京100850 [2]解放军总医院第一附属医院 [3]北京大学第一医院

出　　处：《中华放射医学与防护杂志》2009年第6期580-582,共3页Chinese Journal of Radiological Medicine and Protection

基　　金：全军医药卫生科研基金（06J021）;国家重大科学研究计划（2007CB914603）

摘　　要：目的考察Gadd45基因在健康人群个体间表达的差异，评估Gadd45发展成为一种新的辐射生物剂量计的可行性。方法采集20例健康献血员血液样本，提取白细胞RNA进行反转录，以β-actin作为内对照基因，建立实时定量聚合酶链式反应（PCR）方法，并以此方法探讨人群中Gadd45基因表达水平的差异。结果20例受试者血液标本中的Gadd45表达水平之间存在较大差异．且其表达水平与性别无关。结论放射诱导基因Gadd45的表扶可能不适合做牛物剂量计。Objective To investigate the difference of Gadd 45 expression in healthy population, and to evaluate the possibility of developing Gadd 45 expression into a new radiation bio-dosimeter. Methods Twenty blood samples from healthy population were collected and the total RNA was extracted from white blood cells and reverse transcribed into cDNA. The method of real-time PCR was used to detect the different level of Gadd 45 expression in human population, with β-actin as an internal control. Results There was a significant difference in Gadd 45 expression among 20 subjects. In addition, the difference was irrelevant to the sex. Conclusion The expression of irradiation-inducible gene Gadd 45 might not be a good bio-dosimeter.

关 键 词：Gadd 45 生物剂量计 基因表达 

分 类 号：R144[医药卫生—公共卫生与预防医学]