机构地区:[1]广西医科大学第一附属医院耳鼻咽喉头颈外科,530021
出 处:《中华放射医学与防护杂志》2009年第6期590-594,共5页Chinese Journal of Radiological Medicine and Protection
基 金:广西壮族自治区教育厅科研项目(桂教科研[2003]22号)
摘 要:目的探讨经圆窗膜局部应用地塞米松对放射性内耳损害的保护作用,为头颈部肿瘤放疗所致内耳损害的预防和治疗提供理论依据。方法选取130只豚鼠随机分为4组:(1)地塞米松组(45只):对豚鼠右耳进行70Gy^60Coγ线照射后,经圆窗膜给地塞米松1次;(2)单纯照射组(45只):对豚鼠右耳进行70Gy^60Coγ线照射;(3)盐水对照组(30只):对豚鼠右耳进行70Gy^60Coγ线照射后,经圆窗膜给生理盐水1次;(4)健康对照组(10只):不做任何处理。前3组动物在实验前、照射(给药)后第3天、第2周、第2个月后,分别检测听觉脑干反应(ABR),各组动物均观察中耳黏膜,铺片及扫描电镜观察耳蜗毛细胞的形态学变化。结果ABR阈值正常值为(8.2±2.8)dB,经照射后的3组实验动物ABR阈值均较正常值升高并呈随时间推移逐渐升高的趋势。ABR阈值在第3天、第2周和第2个月的时间段,地塞米松组分别是:(24.0±14.1)、(27.1±9.9)和(38.0±15.1)dB;单纯照射组分别是:(24.5±13.5)、(39.5±15.4)和(57.2±18.4)dB;盐水对照组分别是:(27.0±14.6)、(42.8±13.5)dB(本组仅观察第3天和第2周)。其中,地塞米松组在术后第2周和第2月ABR闯值较其他两组降低,差异具有统计学意义(P〈0.05)。在基底膜铺片和扫描电镜下观察中,健康对照组外毛细胞缺失为(8.0±2.7)个,内毛细胞缺失为(3.7±1.2)个。照射后的3组动物的内、外毛细胞的缺失数量均有明显增加,且外毛细胞多于内毛细胞。各组均表现为随时间推移缺失数逐渐增加的趋势。在术后第2周和第2个月,地塞米松组比单纯照射组的外毛细胞缺失个数减少,分别为(30.7±7.6)与(60.3±14.5)和(67.3±7.0)与(100.0±5.3),两者的差异均有统计学意义(P〈0.05)�Objective To investigate the protective effect of dexamethasone applied to round window membrane (RWM) against radiation-induced inner ear damage in guinea pigs, in order to provide the evidence for preventing and treating radiation-induced inner ear damage. Methods 130 guinea pigs were randomly divided into four groups. ( 1 ) Dexamethasone group( n = 45) : The guinea pigs were injected with dexamethasone through an intact RWM on fight ear for one time after being treated with 70 Gy ^60 Co γ irradiation. (2) Irradiation group( n = 45 ) : The guinea pigs were treated with 70 Gy ^60 Co γ irradiation. ( 3 ) Saline group ( n = 30 ) : Applying of normal saline through an intact RWM on right ear for one time after being treated with 70 Gy ^60 Co γ irradiation. (4) Control group( n = 45) : Auditory brainstem response (ABR) was tested before the 3 rd day, the second week and the second month after irradiation (treatment) in the first three groups. The animals were sacrificed fight after the ABR test and the middle ear mucosa was observed simultaneously. The hair cells were observed by surface preparation of the basilar membrane and scanning electron microscope (SEM). Results The normal ABR threshold is (8.2 ± 2.8) dB. The ABR thresholds increased in those of the three groups treated with irradiation and were rising with time. At the 3rd day, the second week and the second month, ABR thresholds of the dexamethasone group were (24.0 ± 14.1 ), (27.1 ± 9.9) and (38.0 ± 15.1 ) dB, respectively, while the irradiation group were (24.5 ± 13.5), (39.5 ± 15.4) and (57.2 ± 18.4) dB. The saline group were (27.0 ± 14.6) and (42.8 ± 13.5) dB, respectively. ABR thresholds of the dexamethasone group at the second week and the second month both decreased compared with those of the irradiation group and saline group( P 〈 0.05) . In the control group, the outer hair cell lost by 8.0 ± 2.7 and inner hair cell by 3.7 ± 1.2. The los
分 类 号:R764[医药卫生—耳鼻咽喉科]
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...