γ-IFN对兔盆腔常规分割照射后直肠放射性损伤及纤维化的影响  

Effect of the γ-IFN on radiation damage and radiation fibrosis of the rabbit rectum after cavitas pelvisrute fractionated irradiation

作　　者：杨蕴一[1] 张丽[1] 王娟[1] 赵恒芳[1] 代丽[1] 刘孜[1] 

机构地区：[1]西安交通大学医学院第一附属医院,710061

出　　处：《中华放射医学与防护杂志》2009年第6期595-598,共4页Chinese Journal of Radiological Medicine and Protection

摘　　要：目的探讨γ-IFN对兔盆腔常规分割照射后直肠放射性损伤及纤维化的影响。方法建立兔放射性直肠损伤的动物模型，52只新西兰兔分为常规照射组、γ-IFN组及正常对照组，除正常对照组外其余兔盆腔均接受6MVX线照射。γ-IFN组从照射后第5天给予γ-IFN 250000U／kg，1次／周。常规照射组给予5ml／kg的生理盐水。照射后4、8、12和16周处死兔，取其照射范围内的直肠。用HE染色、原位杂交染色及免疫组织化学方法分析γ-IFN对于放射性直肠损伤及纤维化影响。结果照射后4、8和12周γ-IFN组的放射性直肠损伤与常规照射组比较，差异均有统计学意义（P〈0．05），16周差异无统计学意义（P〉0．05）。TGF-β1mRNA原位杂交γ-IFN组直肠黏膜的TGF-β1mRNA表达在照射后4、8、12和16周的放射性直肠损伤与常规照射组和正常对照组比较差异均有统计学意义（P〈0．05）。Ⅲ型胶原免疫组织化学结果显示γ-IFN组与常规照射组和正常对照组比较差异有统计学意义（P〈0．05）。结论γ-IFN可抑制照射后直肠黏膜TGF-β1mRNA的升高，降低Ⅲ型胶原的表达，减轻放射性直肠损伤及纤维化的形成。Objective To investigate the effect of γ-IFN on the radiation-induced injury and fibrosis of the rabbit rectum after the fractionated irradiation to pelvic cavity. Methods The animal model of radiation rectum injury was established. 52 New Zealand rabbits were divided into 3 groups as the normal control group, the conventional radiation group, and γ-IFN group. Except the normal control group, the pelvic cavity of the two other groups were irradiated with 6 MV X-rays. At 5 d post-irradiatio the γ-IFN group was administered with 250 000 U/kg of γ-IFN once a week, while the conventional radiation group with 5 ml of saline solution. The rabbits were executed after 4,8,12 and 16 weeks, and the recta in the irradiated field were taken out. HE dyeing, in-situ hybridization and immuno-histochemical methods were used to analyze the radiation-induced rectrum injury and fibrosis. Results At 4,8, and 12 weeks post-irradiation, the radiation rectum injury and fibrosis were significantly different between the conventional radiation group and γ-IFN group（ P 〈 0.05 ）, but not at 16 weeks （P 〉 0.05）. At 4, 8, 12 and 16 weeks post-irradiation, the expression of TGF-β1 mRNA in-situ hybridization in the γ-IFN group were significantly different compared to the conventional radiation group and the normal control group （ P 〈 0.05） . The immuno-histoehemical results of Col m showed the significant difference among the γ-IFN group and both the normal control group and conventional radiation group （P 〈 0.05 ）. Conclusions γ-IFN could reduce the radiation rectum injury, suppress the expression of TGF-β1 mRNA in the rectum mucosa, inhibit the expression of Col Ⅲ , and delay the formation of radiation-induced fibrosis.

关 键 词：Γ-IFN 放射性直肠损伤 放射性直肠纤维化 TGF—β1 Ⅲ型胶原 兔 

分 类 号：R144[医药卫生—公共卫生与预防医学] R818