内源性大麻素受体1在肥胖大鼠胰腺表达的实验研究  

An experimental study on endocannabinoid receptor I expression in pancreas of high-fat-diet induced obese rats

作　　者：闫晓洁[1] 秦贵军[1] 樊大贝[1] 马晓君[1] 张颖辉[1] 

机构地区：[1]郑州大学第一附属医院内分泌科,450052

出　　处：《中华内分泌代谢杂志》2009年第6期638-641,共4页Chinese Journal of Endocrinology and Metabolism

基　　金：国家自然科学基金（30650005）

摘　　要：高脂饲料喂养20周的sD肥胖大鼠呈胰岛素抵抗特征，免疫荧光双标记显示内源性大麻素受体1（CB1R）主要表达于胰岛D细胞，免疫组化和实时定量PCR结果显示，高脂喂养组胰腺CB1R蛋白（6002．8±855．5vs3712．8±769．6，P〈0．01）及mRNA（对照组的16．7倍，P〈0．05）表达均高于对照组，提示肥胖时胰岛β细胞CB1R表达量上调可能导致胰岛素抵抗。Obese rats manifested the characteristics of insulin resistance after high-fat-diet feeding for 20 weeks. Double immunofluorescence showed that endocannabinoid receptor 1 （CB1R）was greatly expressed in pancreatic β-cells. The results of immunohistochemistry and real-time quantitative PCR revealed that CB1R protein （6 002.8±855.5 vs 3 712.8±769.6 ,P〈0.01 ） and mRNA（ 16.7 folds of control group,P〈0.05 ） expressions in high-fat group were higher than those of control group, suggesting a role of CB1R in high-fat-diet induced insulin resistance.

关 键 词：肥胖症 内源性大麻素受体1 大鼠 Sprague—Dawley 胰岛 间接双重免疫荧光染色 

分 类 号：R589.2[医药卫生—内分泌]