整合素β_1在人输卵管及输卵管妊娠黏膜上皮中的表达  

Expression of Integrin β_1 in both Human Oviductal Tissue and Tubal Ectopic Pregnant Tissue

作　　者：杨筱祎[1] 朱伟杰[1] 徐建平[2] 黎青[2] 

机构地区：[1]暨南大学生命科技学院生殖免疫研究所,广州510632 [2]暨南大学附属第一医院妇产科,广州510630

出　　处：《生殖与避孕》2009年第12期787-790,共4页Reproduction and Contraception

摘　　要：目的:检测整合素β1在人输卵管和输卵管妊娠黏膜上皮中的表达。方法:29例人输卵管组织标本取自因子宫肌瘤、子宫腺肌症等行全子宫加附件切除术的患者。输卵管标本包括峡部、壶腹部和伞部,按子宫内膜组织学分期将标本分为增生早期组(n=8)、增生中-晚期组(n=6)、分泌早期组(n=7)和分泌中-晚期组(n=8)。17例输卵管妊娠组织标本取自输卵管壶腹部妊娠的患者,标本分为种植部位组(n=17)和非种植部位组(n=17)。应用免疫组织化学法检测整合素β1在上述各组黏膜上皮中的表达。结果:整合素β1表达于人输卵管黏膜上皮纤毛细胞和分泌细胞的胞质,其中纤毛细胞的纤毛亦有表达。输卵管峡部及壶腹部黏膜的整合素β1表达水平,不同时期组间的比较无显著性差异(P>0.05);在输卵管伞部,分泌早期组的表达水平显著高于增生早期、增生中-晚期及分泌中-晚期组(P<0.05);在月经周期同一时期,输卵管峡部、壶腹部及伞部间的比较无显著性差异(P>0.05)。在输卵管妊娠黏膜,种植部位的滋养层细胞和蜕膜细胞的胞质可见阳性表达,种植部位的整合素β1表达水平显著高于非种植部位(P<0.05);种植部位和非种植部位的表达水平均显著高于分泌期壶腹部(P<0.05)。结论:整合素β1在分泌早期输卵管伞部黏膜的表达水平升高,在输卵管妊娠种植部位黏膜中表达水平亦升高。Objective： To investigate the expression of integrin β1 in both human oviductal tissues during menstrual cycle and tubal ectopic pregnant tissues. Methods： Samples of oviductal tissue （n=29） were obtained from women undergoing abdominal hysterectomy with adnexectomy for benign disease such as uterine leiomyoma, adenomyosis in the pelvic cavity. These samples included isthmus, ampullae and umbrella, and were divided into the early proliferative stage group （n=8）, the mid-late proliferative stage group （n=6）, the early secretory stage group （n=7）, and the mid-late secretory stage group （n=8） based on their endometrium histological stage. Samples of tubal ectopic pregnant tissues （n=17） were divided into the implantation site group （n=17） and the non-implantation site group （n= 17）. Immunohistochemical method was used to determine the expression level of integrin β1 in the tissues. Results： Expressions of integrin 131 were observed in the cytoplasm of ciliated cells and secretory cells of oviductal tissues. The positive staining was also shown in cilia of ciliated cells. In epithelial cells of the isthmus and the ampullae, no difference was found on expression of integrin β1 in the cycle （P〉0.05）. In umbrella epithelial cells, the expression of integrin β1 in the early secretory stage group had higher level than that of the early proliferative stage group, the mid-late proliferative stage group, or the mid-late secretory stage group, respectively （P〈0.05）. In the same phase of the cycle, the expression among the isthmus, the ampullae and the umbrella showed no change （P〉0.05）. For tubal ectopic pregnant tissues, the expression in the implantation site group had higher level than that of the non- implantation site group or the secretory stage group of ampullae （P〈0.05）. Conclusions： Epithelial cells of the umbrella in the early secretory stage would have higher integrin β1 level. The implantation site of tubal pregnant tissues would also have higher

关 键 词：整合素Β1 输卵管 输卵管妊娠 

分 类 号：R714.221[医药卫生—妇产科学]