WHBE兔网织红细胞计数与分群测定  

Measurement of Reticulocytes and Its Subpopulation in WHBE Rabbits

作　　者：周卫民[1] 潘永明[1] 陈民利[1] 王辉[1] 林琳[1] 王德军[1] 

机构地区：[1]浙江中医药大学动物实验研究中心／比较医学研究中心,杭州310053

出　　处：《实验动物与比较医学》2009年第6期411-414,共4页Laboratory Animal and Comparative Medicine

基　　金：浙江省公共科技基础平台项目（Dwpt-bz-12）;浙江省卫生高层次创新人才培养工程项目

摘　　要：目的测定和比较分析WHBE兔、日本大耳白兔和新西兰白兔的网织红细胞计数与分群的变化。方法选择WHBE兔、日本大耳白兔、新西兰白兔各40只，雌雄各半，耳动脉采血，应用Bayer ADVIA 120五分类血球分析仪测定实验兔网织红细胞参数。结果①性别因素对WHBE兔RDWr、HDWr、CHDWr、RDWm、CHDWm、RDWg、CHDWg等有显著差异（P〈0．05，P〈0．01）；②WHBE兔的网织红细胞参数与日本大耳白兔和新西兰白兔存在着显著差异，如WHBE兔的网织红细胞参数均明显低于日本大耳白兔、新西兰白兔（P〈0．01）；③与人的血液学生理指标比较，发现WHBE兔的网织红细胞生成指数与人类接近。结论WHBE兔网织红细胞计数与分群有其自身的特性，有可能作为人类贫血疾病等研究的模型动物。Objective To determine and comparative analysis of reticulocytes and its subpopulation in WHBE rabbits, Japanese White rabbits and New Zealand White rabbits. Methods Fourty in each of WHBE rabbits, Japanese White rabbits and New Zealand White rabbits, half male and female, were used. The anticoagulant blood samples from the central artery of ear, and then reticulocytes parameters indexes were determined by Bayer ADVIA 120 automatic blood analyzer. Results （1） Gender factor has significant influences on RDWr, HDWr, CHDWr, RDWm, CHDWm, RDWg, CHDWg indexes in WHBE rabbits （P〈0.05, P〈0.01）; （2） Reticulocytes parameters in WHBE rabbits had significant differences between Japanese White rabbits and New Zealand White rabbits, for example, Retic% was lower in WHBE rabbits than that of Japanese White rabbits and New Zealand White rabbits（P〈0.01）; （3）RPI in WHBE rabbits was similar to that of human being, compared with human reference. Conclusion WHBE rabbit has its own reticulocytes and its subpopulation characteristics, and may be used as model animal for research on anemia disease etc. in human.

关 键 词：WHBE兔 日本大耳白兔 新西兰兔 网织红细胞 

分 类 号：Q95-33[生物学—动物学]