淋病奈瑟菌感染模型中aniA及norB基因的表达及其临床意义  

The Expression of aniA and norB Genes in Gonococcal Infection Model and Its Clinical Significance

作　　者：陈嵘祎[1,2] 陈宏翔[1] 吴志洪[1] 李娟[1] 许莉[1] 俞莺[1] 谭明[1] 涂亚庭[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院皮肤性病科,湖北武汉430022 [2]广东医学院附属医院皮肤科,广东湛江524001

出　　处：《中国皮肤性病学杂志》2009年第12期771-773,789,共4页The Chinese Journal of Dermatovenereology

基　　金：国家自然科学基金(编号:30700717)

摘　　要：目的研究aniA及norB基因在淋病奈瑟菌(淋球菌)感染模型中的表达及其临床意义。方法分离正常人外周血中性粒细胞(PMN),将其分为对照组(A组)及淋球菌感染组(B组)。在0,3,8,12h分别以实时荧光定量PCR测定各组PMN中iNOS mRNA及淋球菌aniA,norB mRNA的表达,用镉还原法检测各组细胞培养液中的一氧化氮(NO)浓度。结果B组中iNOS,aniA,norB mRNA的表达及水平均较A组升高;NO浓度在8h达到高峰,以后逐渐下降(P<0.05)。而A组中各时间段iNOS,norB mRNA的表达及NO浓度无明显变化。在淋球菌刺激早期B组norB表达与N0成正相关(r_s=0.862,P<0.01),而在norB上调后N0浓度逐渐下降。结论淋球菌感染中aniA,norB基因的上调,可能与该菌适应体内微氧环境实现免疫逃逸并致病有关。Objective To investigate the pathogenic effects of aniA and norB gene on PMN inflammatory responses to Gonococcal infection and its clinical significance. Methods Isolated from heparinized peripheral blood of healthy men, PMN were divided into two groups： control group（A） and gonococcus infection group（B）. The expressions of iNOS, aniA and norB mRNA transcript and NO concentration were detected by real-time RTPCR and method of chromium reduction at various time points（0,3,8,12h） respectively. Results The expressions of iNOS, aniA, norB mRNA transcript and NO concentration increased obviously in group B, and NO concentration reached their peak at 8h （P 〈 0.05 ） while no significant changes could be found in the expressions of iNOS, norB mRNA transcript and NO in group A （P 〉 0.05 ）. There was a positive correlation between norB and NO in group B at earlier period （ rs = 0. 862 ,P 〈 0.01 ） and then NO concentration fell off after norB expresstion upgraded. Conclusion Gonoccocus aniA and norB genes＇ up-regulation, which is important for gonococcal adaptation to an anaerobic environment during gonococcal infection, may have implications in immune evasion and immunomodulation in the human host.

关 键 词：淋球菌 aniA norB 

分 类 号：R759.2[医药卫生—皮肤病学与性病学]