三氧化二砷下调JAK3表达并抑制淋巴细胞增殖  

Arsenic Inhibits Proliferation of Lymphocytes Via Down-regulating JAK3 Expression

作　　者：毕新岭[1] 李瑾[1,2] 顾军[1] 田中伟[3] 

机构地区：[1]第二军医大学长海医院皮肤科,上海200433 [2]海军航空工程学院青岛分院门诊部,山东青岛266001 [3]河南新乡医学院第一附属医院皮肤科,河南新乡4530032

出　　处：《中国皮肤性病学杂志》2009年第12期787-789,共3页The Chinese Journal of Dermatovenereology

基　　金：上海市科委基础医学重点项目(08JC1406800);杨森科学基金(2008)

摘　　要：目的研究三氧化二砷对淋巴细胞增殖和JAK3表达的影响。方法以分离培养的外周血淋巴细胞为对象,不同浓度砷剂处理后,采用3H-TdR方法检测淋巴细胞增殖;并进一步探讨其影响淋巴细胞增殖的机制,用Realtime-PCR方法研究细胞JAK3表达情况。结果0.01,0.1,1μmol/L砷剂组,摄入的同位素活性不同程度降低,其中0.01μmol/L砷剂组同对照组相比,差异无统计学意义(P>0.05),而0.1和1μmol/L砷剂组与对照组相比,差异均有统计学意义(P<0.05);Realtime-PCR结果显示:增殖抑制的淋巴细胞组,JAK3 mRNA水平下调。结论一定浓度的砷剂可下调JAK3的表达,抑制淋巴细胞增殖,从而影响机体的免疫功能。Objective To study the effects of arsenic on cell proliferation and expression of JAK3 in lymphocytes. Methods Human peripheral blood lymphocytes were separated and cultured in vitro. , 3H-TdR method was used to detect proliferation of lymphocytes treated with various concentration of arsenic. The expression of JAK3 in lymphocytes was assessed by Realtime-PCR method to explore the mechanism of lymphocytes proliferation. Results In the groups treated with arsenic trioxide （0.01, 0.1, and 1μmol/L） ,the amount of incorporated 3H-TdR decreased. There was no significant difference between 0.01 1μmol/L arsenic group and the control group（P 〉0.05 ）. Significant differences were noted between 0. 1, 1μmol/L arsenic groups and the control group（P 〉0.05）Realtime-PCR analysis showed that levels of JAK3 mRNA were down-regulated in lymphocytes which were inhibited. Conclusion Certain concentration arsenic led to down-regulation of JAK3 and inhibited lymphocytes proliferation so as to affect the immune function.

关 键 词：砷剂 淋巴细胞 细胞增殖 JAK3 

分 类 号：Q253[生物学—细胞生物学]