文蛤金属硫蛋白基因的克隆与分析  

Analysis on Cloning and Cequence Characteristics of Metallothionein Gene in Dard Clam Meretrix meretrix

作　　者：高祥刚[1] 赫崇波[1] 李云峰[1] 王健[1,2] 朱丹[1,2] 刘卫东[1] 鲍相渤[1] 

机构地区：[1]辽宁省海洋水产科学研究院,辽宁省海洋水产分子生物学重点实验室,辽宁大连116023 [2]辽宁师范大学生命科学学院,辽宁大连116029

出　　处：《水产学杂志》2009年第4期8-11,共4页Chinese Journal of Fisheries

基　　金：辽宁省科学技术计划重大、重点项目(项目编号:2008203001);辽宁省海洋与渔业科研计划项目(200801);国家海洋公益性行业科技专项(编号:200805037)

摘　　要：金属硫蛋白是一种普遍存在于生物体内的低分子量、半胱氨酸含量丰富、易于被外界刺激诱导的金属结合蛋白。采用表达序列标签(EST)法,首次获得了文蛤金属硫蛋白(Mm-MT)的全长cDNA序列,序列全长657bp,开放阅读框长度为231bp,可以编码76个氨基酸。编码的氨基酸中半胱氨酸含量丰富,达27.6%,含有软体动物等无脊椎动物金属硫蛋白的特征序列。序列特征分析表明,该序列具备金属硫蛋白的典型特征,是金属硫蛋白家族成员。Metallothioneins （MT） are low molecular weight, cysteine-rich, inducible metal-binding proteins found in a variety of organisms. MTs have many important biological functions acted as a protective role against excess reactive heavy metal ions, free radical scavengers and reservoir of essential metals that can be donated to other metalloproteins. The full-length eDNA sequence of metal- lothionein （named as Mm-MT） gene in the hard clam （Meretrix meretrix） was identified from M. meretrix eDNA library using ex- pressed sequence tag （EST） method. The full-length eDNA of Mm-MT is of 657 bp and an open reading frame （ORF） of 231 bp en- coding a polypeptide of 76 amino acids. The contents of cys in the -Mm-MT amino acid sequence is 27.6%. Mm-MT is characterized by the conserved features of invertebrate＇s MTs. The result of sequence analysis indicates that Mm-MT is the member of metallothionein family. This research will provide basic information for evolution and function study of marine shellshef metallothionein.

关 键 词：文蛤 金属硫蛋白基因 EST CDNA文库 

分 类 号：Q78[生物学—分子生物学]