烷基卤脱卤酶作用机制的研究新进展  

New Advance in Study of Hydrolytic Dehalogenation Catalyzed by Haloalkane Dehalogenase

作　　者：张玉花[1] 陈德展[1] 

机构地区：[1]山东师范大学化学化工与材料科学学院,山东济南250014

出　　处：《山东化工》2009年第12期20-23,27,共5页Shandong Chemical Industry

摘　　要：烷基卤脱卤酶的催化脱卤反应具有重要的合成价值和治理环境污染方面的巨大潜力。随着酶晶体结构的确定,它的催化作用机制逐渐被研究。目前,催化循环机制在很多方面仍然存有争议。本文对烷基卤脱卤酶催化脱卤机制的研究新进展进行了综述,以期对脱卤机制进行进一步研究,不断提高酶的生物催化活性和拓宽底物范围,为工业上生物催化难降解有机卤化物提供理论依据。Haloalkane dehalogenases （EC 3.8. 1.5） comprise such a group of microbial enzymes that catalyze the conversion of haloalkanes into the corresponding alcohols and halide anions. Hydrolytic dehalogenation catalyzed by haloalkane dehalogenase is important to chemosynthesis and has quite potential use in environmental pollution. Recently, X - ray structures of three different bacterial haloaIkane dehalogenases have been solved and the catalytic properties of these enzymes have therefore been subjected to detailed investigations. However, some mechanistic issues are still uncertain. In this review, recent results from mechanistic studies are discussed to attempt to enhance the catalytic activity and to expand the substrate range, which can further promote the degradation of some remaining problem compounds and the development of dehalogenases for use in industrial biocatalysis.

关 键 词：烷基卤脱卤酶 1.2-二氯乙烷 脱卤 生物降解 

分 类 号：TQ426.97[化学工程]