Patatin启动子驱动的人胰岛素原基因的植物表达载体的构建  

Construction of Human Proinsulin Gene Plant Expression Vector Controlled by Patatin Promoter

作　　者：马建忠[1] 马雪青[1] 王永刚[1] 宋国伟[1] 周贤靖[1] 王倩[1] 

机构地区：[1]兰州理工大学生命科学与工程学院,甘肃兰州730050

出　　处：《中国马铃薯》2009年第6期332-337,共6页Chinese Potato Journal

基　　金：甘肃省自然基金马铃薯块茎生产天然人胰岛素的研究资助(3ZS062-B25-023)

摘　　要：使用马铃薯的偏爱密码子设计合成人胰岛素原基因,以植物表达载体pCAMBIA1301为基础,构建由patatin启动子调控的人胰岛素原基因植物表达载体p1301Ph,并将导入根癌农杆菌LBA4404中,经PCR鉴定证明,此基因已整合到农杆菌Ti上。表达载体的建立为日后转基因工作奠定了基础。Human proinsulin gene was designed and synthesized with the potato partial codon. Plant expression vector p1301Ph was derived from pCAMBIA 1301, with human proinsulin gene being controlled by patatin promoter. Then the recombination plasmid was introduced into Agrobacterium LBA4404. It was proven that the vector was integrated into LBA4404. This study provides a foundation for the transgenic of plants.

关 键 词：patatin启动子 人胰岛素原基因 植物表达载体 

分 类 号：S532[农业科学—作物学] Q578[生物学—生物化学]