海南黎族人血小板1—17抗原基因与随机输血不配合率研究  

Investigation of the polymorphism of HPA-1-17 gene and the platelet transfusion refractoriness in Li population on Hainan island,in China

作　　者：夏兰[1] 蔡兴权[1] 陈鑫萍[1] 符小玲[1] 符生苗[1] 符有畅[1] 吴丽妮[1] 于柏峰[1] 陈德娟[1] 符文锋[1] 

机构地区：[1]海南省人民医院输血科,海南海口570311

出　　处：《中国输血杂志》2009年第11期890-893,共4页Chinese Journal of Blood Transfusion

基　　金：海南省自然科学基金资助(项目批准号:806113)

摘　　要：目的调查海南岛黎族人群血小板抗原(HPA)-1—17等位基因多态性及其特点,评估其在随机输血中血小板输注无效的风险。方法采用PCR-SSP方法对海南岛180名黎族人群做HPA-1—17基因分型检测>计算各系统对偶抗原不配合率。结果在海南黎族人群的HPA-1—17系统中,呈多态性分布的等位基因及其频率为HPA-2a(0.9972)、2b(0.0028),HPA-3a(0.4889)、3b(0.5111),HPA-5a(0.9667)、5b(0.0333),HPA-6a(0.9972)、6b(0.0028),HPA-15a(0.4250)、15b(0.5750);其余HPA-1、4、7—14、16、17等位基因均呈单线性分布。在HPA-3、15等位基因中出现bb纯合子基因型,频率分别为0.2834和0.3667;其余系统均未见bb纯合子基因型。随机输血中,海南岛黎族人群HPA不配合的发生率依次为:HPA-3(37.49%)、-15(36.93%)和-5(6.23%)。结论揭示了海南岛黎族人HPA-1—17基因型和等位基因频率的分布及特点;立足人群的随机血小板输注,只需检测供、受者HPA-2,-3、-5和-15基因相合,就可基本达到血小板匹配性输注。Objective To investigate the allele polymorphism and characteristic of HPA-1-17 genotyping of Li ethnic on Hainan island, and to evaluate the HPA mismatched ratio between donor and recipient in randomly chosen eases of blood transfusion, in order to investigate the platelet transfusion refractoriness in Li population. Methods Genotyping tests of A-1--17 allele of the blood sample from 180 Lis were conducted with PCR-SSP. Results A total of 180 blood samples were genotyped successfully in HPA genetic sys tern, among which 0. 9972 were HPA-2a, 0. 0028 were HPA-2b, 0. 4889 were HPA-3a,0. 5111 were HPA-3b, 0. 9667 were HPA-5a,0. 0333were HPA-5b, 0. 9972 were HPA-6a, 00028 were HPA-6b,0. 4250 were HPA-15a, 0. 05750 were HPA-15b, and they all showed polymorphism. But polymorphism was not detected out in other allelie genes, such as HPA- 1, -4, -7--14, -16, and -17. The bb homozygous were detected in two alleles, among which 0. 2834 was HPA-3 and 0. 3667 was HPA-15, but it wasnt detected in the others systems. In the randomly chosen eases of blood transfusion, the mismatch rate of HPA-3 , HPA-15 and HPA-5 was 37. 49%, 36.93% and 6. 23%, respectively. Conclusion This re- search revealed the distribution of HPA gene in Li population on Hainan island, and found the allelie frequency and thier characteristics. Aeeordin8 to our findings, HPA-2, HPA-3, HPA-5 and HPA-15 genes between donor and recipient should be matched in order to avoid platelet transfusion refraetoriness.

关 键 词：黎族 血小板抗原 HPA基因频率 血小板输注 不配合率 海南岛 

分 类 号：R457.11[医药卫生—治疗学] R331.1[医药卫生—临床医学]