一株香蕉拮抗内生细菌的分离及鉴定  

Isolation and Identification of One Strain of Antagonistic Endophytic Bacteria from Banana

作　　者：吕恒玉[1] 张科立[1] 刘先宝[1] 蔡吉苗[1] 黄贵修[1] 

机构地区：[1]中国热带农业科学院环境与植物保护研究所,农业部热带农林有害生物入侵监测与控制重点开放实验室,海南儋州571737

出　　处：《热带农业工程》2009年第5期26-30,共5页Tropical Agricultural Engineering

基　　金：科技部国家科技支撑计划项目子课题资助(No.2007BAD48B05-5)

摘　　要：从健康的香蕉组织内分离获得22株内生细菌,从中获得1株对香蕉枯萎病菌(Fusarium oxysporum f.sp.cubense)1号与4号小种具有抑制作用的拮抗菌(编号为BEB9),经形态学、生理生化及16S rDNA序列鉴定等方面研究,确认为伯克霍尔德菌。研究还发现NA和YE培养基是其抑菌作用最适培养基。Twenty two endophytic bacterial strains were isolated from the healthy tissues of banana, of which 1 strain named BEB9 was screened and showed inhibitory activities against races 1 and 4 of Fusarium oxysporum f sp cubense. This strain was identified as Burkholderia based on its morphological, physiological, and biochemical characteristics and the 16S rDNA sequences analysis, and NA and YE cultural media were optimum for antagonistic culture of this strain.

关 键 词：香蕉 内生细菌 洋葱伯克霍尔德菌 

分 类 号：S668.1[农业科学—果树学]