部分烟区烟草病毒病病原检测及复合侵染分析  

Virus Detection of Tobacco Viral Disease and Analysis of Viruses Mixed-infection in Some Tobacco Growing Areas

作　　者：青玲[1,2] 牛颜冰[3] 吴楚钊 肖崇刚[1] 周常勇[1,2] 

机构地区：[1]西南大学植物保护学院植物病害生物学重庆市高校级重点实验室、重庆市北碚天生路1号,400716 [2]中国农科院柑橘研究所、重庆市北碚歇马镇柑桔村15号,400712 [3]山西农业大学、太原市兴农街1号,030801 [4]饶平出入境检验检疫局、广东省饶平黄冈镇龙眼城汕汾西路北58号,515700

出　　处：《烟草科技》2009年第12期58-64,共7页Tobacco Science & Technology

基　　金：国家青年科学基金项目“烟草曲茎病毒病害复合体种群遗传结构及变异研究”(30700524);西南大学博士后基金资助项目“我国部分地区烟草和蔬菜病毒病主要病毒种类的检测及鉴定”(207001)

摘　　要：为明确主要烟区烟草病毒病的主要病毒种类,采用抗原直接包被ELISA法对重庆、四川、云南、贵州、河南、陕西和甘肃等省市部分烟区采集的266个烟草病毒病样品进行了检测。结果表明,样品中共检测到马铃薯Y病毒(Potato virus Y,PVY)、烟草花叶病毒(Tobacco mosaic virus,TMV)、黄瓜花叶病毒(Cucumber mosaic virus,CMV)、番茄花叶病毒(Tomato mosaic virus,ToMV)及芜菁花叶病毒(Turnipmo-saic virus,TuMV)等5种病毒。其中PVY的侵染最普遍,总检出率达75.56%;TMV、CMV、ToMV和TuMV的总检出率分别为65.41%、57.14%、36.84%和34.59%,其中,ToMV和TuMV仅在云南、四川、贵州和重庆样品中检测到。在257个阳性样品中有97个样品受CMV、TMV或PVY单独侵染,其检出率分别为7.39%、13.23%和17.12%;其余样品均受2种以上病毒复合侵染,其中55.63%的样品中检测到5种病毒复合侵染。田间烟草样品表现出复杂的病毒病症状类型。这些结果说明田间烟草病毒复合侵染现象严重,PVY已成为当前我国烟区的优势毒原。In order tO investigate the main viruses causing tobacco viral diseases in main tobacco-growing areas, 266 tobacco leaf samples with viral disease symptoms collected from part of the tobacco-growing areas in Chongqing, Siehuan, Yunnan, Guizhou, Henan, Sha＇ anxi and Gansu were tested by antigen direct coated enzyme-linked immunosorbent assay （ELISA）. Tobacco mosaic virus （TMV）, Cucumber mosaic virus （CMV）, Tomato mosaic virus （ ToMV ）, Turnip mosaic virus （TuMV） and Potato virus Y （PVY） were detected. The infection percentage （75.56%） of PVY was the highest, and that of TMV, CMV, ToMV and TuMV was 65.41% , 57.14% , 36.84% and 34.59% , respectively. ToMV and TuMV were detected only in the samples from Yunnan, Sichuan, Guizhou and Chongqing. Of the 257 positive samples, 97 cases were infected by CMV （7. 39% ）, TMV （13. 23% ） or PVY （17. 12% ） alone. Other positive samples were infected by two or more viruses and about 55.63% of them were infected by all the five viruses. Tobacco samples in the field showed complex viral disease symptoms. These results indicated that viral mixed-infection occurred seriously in field tobacco and PVY has become a dominant virus in some tobacco-growing areas.

关 键 词：烟草 病毒 复合侵染 

分 类 号：S432.41[农业科学—植物病理学]