湖北钉螺空间遗传信息管理系统的设计与构建  

Preliminary study on spatial distribution and genetic variation database of Oncomelania hupensis in China's Mainland

作　　者：李石柱[1] 马琳[2] 王艺秀[2] 王强[1] 胡缨[1] 张仪[1] 周晓农[1] 

机构地区：[1]中国疾病预防控制中心寄生虫病预防控制所世界卫生组织疟疾血吸虫病丝虫病合作中心卫生部寄生虫病原与媒介生物学重点实验室,上海200025 [2]陕西师范大学生命科学学院

出　　处：《中国血吸虫病防治杂志》2009年第6期473-476,F0003,共5页Chinese Journal of Schistosomiasis Control

基　　金：国家自然科学基金(30590373);世界卫生组织热带病研究和培训特别规划项目(970990);科技部重大支撑专项(2003DIA6N009;2005DKA21104;2007BAC03A02);国家传染病防治重大专项(2008ZX10004-011)

摘　　要：目的探讨基于空间分析基础上的湖北钉螺群体遗传变异,构建湖北钉螺空间分布和遗传信息数据库及其管理系统,为相关研究、信息管理和开发利用提供先进的方法和手段。方法采集现场钉螺样本,以逸蚴法鉴定阴性钉螺。利用Microsoft SQL2000建立采集点空间分布数据库、样本数据库和遗传信息数据库,用Visual Basic 6.0建立相应管理系统。结果完成现场钉螺收集,初步建立了10个省73个采集点的数据库,676个记录的样本数据库和部分遗传信息数据库,管理系统实现添加、查询、删除、统计和数据导出导入等功能,界面清晰,操作简便。结论建立的湖北钉螺空间遗传信息管理系统对湖北钉螺空间分布和群体遗传研究具有一定的应用价值,但还需进一步补充和完善相关数据。Objective To construct a spatial genetic database of Oncomelania hupensis and a managing system for spatial distribution and population studies based on landscape genetics theory.Methods The uninfected Oncomelania snails were collected from the field.Collected sites database,specimen database and genetic information database were set up by using Microsoft SQL 2000,and the managing system was set up by using Visual Basic 6.0.Results The Oncomelania snails were collected from the field.The database were consummated with 676 specimens,73 collected sites from 10 provinces,and the database could be used as a series of celerity tools with appending,querying,deleting,statistics and export functions.Conclusion The spatial genetic database and managing system of Oncomelania hupensis can be used to manage snail specimens for different studies with a clear interface and simple operation.It is worthy to renew the database for further spatial genetic studies.

关 键 词：湖北钉螺 空间分布 遗传变异 数据库 

分 类 号：R383.24[医药卫生—医学寄生虫学]