醛固酮合酶基因-344 C/T的多态性与心房颤动的关联研究  

Association of aldosterone synthase gene-344C/T polymorphism with atrial fibrillation

作　　者：张复贵 闵新文[2] 曾秋棠[3] 毛晓波[3] 易桂文[3] 吉庆伟[3] 

机构地区：[1]恩施自治州中心医院心内科,湖北恩施445000 [2]郧阳医学院附属东风医院心内科 [3]华中科技大学附属协和医院心内科

出　　处：《临床心血管病杂志》2009年第12期919-922,共4页Journal of Clinical Cardiology

摘　　要：目的:探讨醛固酮合酶(CYP11B2)基因-344C/T的多态性与心房颤动(Af)的关系。方法:研究对象均来自湖北地区汉族人群,包括120例Af患者,120例非Af者。采用成组配比研究,取静脉血,提取基因组DNA,采用聚合酶链反应-限制性酶切片段长度多态性(PCR-RFLP)分析技术对2组人群CYP11B2基因-344C/T的多态性进行分析。结果:CYP11B2-344CT+CC基因型频率在Af组与对照组之间差异有统计学意义(53.4%∶37.5%,P=0.037),等位基因在2组间亦存在同样的趋势(C/T=28.8%∶19.6%,P=0.019)。单因素非条件Logistic回归分析CT+CC基因型频率患Af的危险性高(OR=1.82,95%CI1.02～3.22,P=0.04),排除混杂因素后,CT+CC基因型与人群患Af的风险呈弱相关(OR=1.73,95%CI0.99～3.02,P=0.056);排除混杂因素后,左房内径与人群患Af的风险总是呈显著相关(OR=8.14,95%CI3.43～19.31,P=0.000)。结论:在湖北地区汉族人群中,CYP11B2-344C/T的点突变与Af的发病呈弱相关性,有可能是Af的遗传危险因素,左房内径的增加与Af的发病呈显著相关。Objective：This study aimed to investigate the relationship between the polymorphism oi the aldo-sterone synthase （CYP11B2） gene --344 C / T and atrial fibrillation. Method：All human came from the Han population in Hubei Province, 120 cases were atrial fibrillation patients, 120 were non-atrial fibrillation. Vein blood were acquired to extracte genomie DNA, and polymerase chain reaction restriction fragment length polymorphism （PCR-RFLP） was used to analyse the polymorphism of the aldosterone synthase （CYPllB2） gene --344C/T analysis in two populations. Result：There was a significant difference between the ease group and control group in CC ＋CT frequencies of genotype CYPllB2 - 344 C/T polymorphism （respectively 53.4% ： 37.5%, P = 0. 037）. There was also the same trend between alleles（C/T=28.8%： 19.6%, P=0. 019）. The crowd carrying CT＋CC genotype suffered from the high risk of AF by single factor non conditional Logistic regression analysis （OR=1.82, 95% CI= 1.02-3.22,P=0.04）, however, after exclusion of confounding factors, there were weak association for the people carrying CT＋CC genotype suffering from the risk of atrial fibrillation （OR= 1.73,95 % CI = 0.99-3.02,P=0. 056）. After exclusion of confounding factors, there were significantly association between left atrial diameter and the risk of suffering from atrial fibrillation（OR = 8.14, 95% CI = 3.43- 19.31, P = 0. 000）. Conclusion：Among the Han population in Hubei Province, CYP11B2 --344 C/T gene polymorphisms is weakly associated with atrial fibrillation, which may also be a risk factor of atrial fibrillation, increase in left atrial diameter is significantly associated with the incidence of atrial fibrillation.

关 键 词：心房颤动 单核苷酸多态性 醛固酮合酶 聚合酶链反应-限制性酶切片段长度多态性 

分 类 号：R541.75[医药卫生—心血管疾病]