^125I粒子组织间植入联合外照射治疗小鼠Lewis肺癌的实验研究  

The experimental study of ^125 I seeds interstitial brachytherapy combined with external beam radio- therapy for Lewis lung cancer in C57BL/6 mice

作　　者：张兴利[1] 陈杰[1] 史建平[1] 吴传峰[1] 周俊东[1] 周华[1] 黄敏[2] 吴锦昌[1] 

机构地区：[1]南京医科大学附属苏州医院肿瘤放疗科,215001 [2]南京医科大学附属苏州医院超声中心,215001

出　　处：《中华核医学杂志》2009年第6期386-390,共5页Chinese Journal of Nuclear Medicine

基　　金：苏州市科学技术局科技计划项目（SZD0632,SS0707）

摘　　要：目的探讨^125I粒子组织间植入联合外照射治疗肺癌的有效性。方法建立C57BL／6小鼠Lewis肺癌（LLC）实体瘤模型后，按完全随机化法分为对照组、单纯^125I粒子组织间植入内放疗组、单纯外放疗组（15Gy）、^125I粒子组织问植入联合外放疗（^125I＋外放疗8Gy）组，每组6只小鼠。分组处理后每3天测量肿瘤体积，15d后处死小鼠并测肿瘤质量，计算抑瘤率，制作肿瘤组织标本，行常规病理学检查，以免疫组织化学法检测肿瘤微血管密度（MVD）的表达。利用单因素方差分析比较组间差异，行SNK法q检验。结果与对照组比较，单纯内放疗组、单纯外放疗组（15Gy）及联合放疗组（^125I粒子＋外放疗8Gy）LLC体积均有不同程度的生长抑制（q=11．06，17．13，16．31，P均〈0．05），单纯外放疗组与联合放疗组最明显（抑瘤率分别为50．9％、48．4％），虽目前两者瘤质量差异无统计学意义（q=0．50，P〉0．05），但联合放疗组抑瘤率高于单纯内放疗组（48．4％与28．6％）。免疫组织化学结果显示4组小鼠肿瘤MVD值分别为（23．33±4．84）、（17．50±3．67）、（11．83±2．14）、（12．67±3．39）个微血管／高倍视野（×200），单纯内放疗组的MVD值低于对照组，且高于联合放疗组，差异有统计学意义（q=3．92和3．25，P均〈0．05），而联合放疗组与单纯外放疗组间的差异无统计学意义（q=0．57，P〉0．05）。结论放疗可通过降低肿瘤MVD值，减少血管生成而抑制肿瘤生长。在达到相同的肿瘤局部控制率的情况下，联合^125I粒子组织间植入照射可有效减少外照射剂量，使周围正常组织器官的吸收剂量减低。Objective The purpose of this study was to investigate the efficacy of ^125 I seeds interstitial brachytherapy combined with external beam radiotherapy for lung neoplasm. Methods Each set of six C57BL/6 mice bearing Lewis tumor was stratified into four groups on day 1 ： vehicle control, ^125 I seeds interstitial brachytberapy alone, external beam radiotherapy （15 Gy） alone, and combination of ^125 I seeds interstitial brachytherapy with external beam radiotherapy （8 Gy ）. After treatment, the volume of the tumors was measured every 3 days. The animals were executed, the weight of tumors was measured, tumor control rate was calculated and pathological examination was performed on day 15. The intratumoral micro-vascular density （MVD） in each group was evaluated by immunohistochemical methods. Tumor vnlume, weight and MVD were compared by one-way ANOVA, student-newman-keuls （SNK）-q analysis. Results Comparing with control group, the other three groups had significant inhibiting effect on the growth of Lewis tumor （q = 11. 06, 17.13, 16.31, all P 〈0.05）. Tumor growth inhibition in external beam radiotherapy alone group and combination group （50.9% and 48.4% respectively） were higher than 1251 seeds interstitial brachytherapy group （ 28.6% ） , while the difference of tumor weight was not significant between the former two groups （ q = 0. 50, P 〉 0. 05 ）. Immunohistocbemlstry analysis showed that the MVD value in the four groups was （ 23.33 ±4.84 ）, （ 17.50 ± 3.67）, （ 1 1.83 ±2.14） and （ 12.67 ±3.39 ） / （ high power field ×200 ） respectively. The MVD value of ^125I seeds interstitial brachytherapy alone group was much lower than that of control group, and higher than combination group （q = 3.92 and 3.25, both P 〈0.05 ）. Additionally, there was no significant difference between external beam radiotherapy alone group and combination group （q = 0. 57, P 〉 0.05 ）.Conclusions Radiotherapy can significantly reduce the MVD values of tumor and inhibit

关 键 词：肺肿瘤 放射疗法 碘放射性同位素 小鼠 

分 类 号：R686[医药卫生—骨科学]