成年大鼠与新生鼠嗅鞘细胞形态学和生长特性的差异研究  

Differentiation of morphology and growth features of olfactory ensheathing cells between adult rat and neonatal rat

作　　者：李伟[1] 肖传国[2] 

机构地区：[1]广西壮族自治区人民医院泌尿外科,南宁市530021 [2]华中科技大学同济医学院泌尿外科研究所,武汉市430022

出　　处：《实用医学杂志》2009年第24期4124-4126,共3页The Journal of Practical Medicine

基　　金：国家重点基础研究发展计划资助项目(编号:2003CB515300)

摘　　要：目的:对成年大鼠和新生鼠嗅鞘细胞的形态学和生长特征的差异进行观察。方法:使用相同培养纯化方法分别培养成年大鼠和新生鼠嗅球表层中的嗅鞘细胞,NGFRp^(75)免疫组化染色鉴定细胞并观察不同时期两者嗅鞘细胞的形态差异和生长速度。结果:均可获得较高纯度的嗅鞘细胞,但两类细胞在生长特征和形态学上略有差异结论:成年大鼠和新生鼠嗅鞘细胞形态学和生长特征有差异。Objective To observe the difference of morphology and growth features of olfactory ensheathing cells （OECs）between adult rat and neonatal rat. Methods OECs of adult rast and neonatal rats were cultured and purified by the same methods. OECs were identified by nerve growth factor receptor p75 （NGFRp^75） immunohistochemical staining, and the difference of morphology and growth speeds of OECs between adult rat and neonatal rat in different stages were observed respectively. Results High purity OECs of both adult rat and neonatal rat could be obtained, but two kinds OECs had slight difference in growth speeds and morphology. Conclusion There are slight difference of morphology and growth features of OECs between adult rat and neonatal rat.

关 键 词：嗅神经 成年大鼠 新生大鼠 形态学 生长特性 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]