血红蛋白浓度的光学测量方法与装置  

Optical measuring methods and apparatus for hemoglobin concentration in whole blood

作　　者：黄博[1] 姚玉峰[1] 姜瑞举 

机构地区：[1]哈尔滨工业大学(威海)机器人研究所,山东威海264209 [2]威海医用高分子集团,山东威海264209

出　　处：《光学精密工程》2009年第12期2893-2898,共6页Optics and Precision Engineering

基　　金：国家863高技术研究发展计划资助项目(No.2007AA042105);威海市科技攻关项目

摘　　要：为了实现全血中血红蛋白浓度的精确测定,研制了便携式血红蛋白浓度光学测量系统,对该系统的测量机理、浓度计算公式、药剂微片、测量装置及控制系统进行了研究。首先,基于叠氮高铁血红蛋白溶液的吸收光谱曲线设计了二次吸收测量机理,并明确了浓度计算公式。然后,设计了供光学测量的微片,对微片进行亲水处理以确保全血毛细吸附,并预填指定配方的药剂,光学容腔的公差可确保光学测量的精度与一致性。最后,给出了测量装置与控制系统的详细方案。该装置可在30～60s内实现未稀释未溶血全血的在线快速测量。临床试验表明,该方法与氰化高铁血红蛋白法相关性可达0.9836,准确性达96.32%。该测定仪具有便携、无毒和在线快速检测等特点,适用于移动采供血、运动员机能评定以及家庭保健等。A portable and accurate optical measuring system for hemoglobin is developed,and its measuring principle, concentration equation, medicament micro-tablet, measurement apparatus and control architecture are also studied. Firstly, the secondary absorb measurement mechanism is designed based on the absorbing spectrum of azide methemoglobin, and the concentration equation is derived. Then the micro-tablet for optical measuring is designed,in which the medicament micro tablet is hydrophilic to insure the whole blood capillary absorption and to achieve the accuracy and consistency of optical measuring. Finally, the measuring mechanism and control system are introduced in detail, which demonstrates that the measuring system can detect the hemoglobin concentration within 30-60 s. Moreover, the clinical trials show that the correlation of the measurement results between the proposed method and the cyaniding methemoglobin （Fe^3＋） method is up to 0. 983 6 and the veracity can be 96.32%. Because of the portability, no contamination and fast test on line, the instrument can be widely used in moving blood collecting, function judging of the athletes and family health cares.

关 键 词：血红蛋白 浓度测量 光谱分析 

分 类 号：TH773[机械工程—仪器科学与技术]