一氧化氮的释放对海马脑片CAl区痫样放电的影响  

EFFECT OF NITRIC OXIDE RELEASE ON EPILEPTIFORM DISCHARGE IN CA_1 AREA OF HIPPOCAMPAL SLICES

作　　者：陆巍[1] 陈刚[2] 程介士 

机构地区：[1]上海医科大学神经生物学教研室 [2]上海医科大学化学教研室,上海200032 [3]医学神经生物学国家重点实验室,上海200032

出　　处：《生理学报》1998年第5期507-513,共7页Acta Physiologica Sinica

基　　金：国家自然科学基金!No39570886

摘　　要：用自制的一氧化氮（NO）敏感电极──Nation-壳聚糖合镍修饰铂电极（Nation-CTS（Ni）-Pt）连续测定了青霉素致痫海马脑片CAl区锥体层神经元NO的释放，并同时观察了NO合酶抑制剂7-nitro-indaole（7-NI）及Nω-nitro-L-arginine（L-NNA）对诱发痫波及NO释放量的影响。研究观察到：（1）在青霉素致痫脑片模型上，诱发的痫波随青霉素浓度的增加而增多，与此同时，NO释放量亦增加并存在剂量反应关系；（2）NO合酶抑制剂7-NI及L-NNA抑制海马脑片CAl区NO的产生的同时，可部分翻转青霉素的致痫作用；（3）NO敏感电极检测线性范围为4．5×10－4～1．0×10－8mol／L，可用于生物组织如脑片的NO释放量测定。结果提示，NO可能有致痫作用；L-NNA及7-NI对癫痫有抑制作用；NO敏感电极的应用为生物体NO的连续实时测定提供了有效的实验工具。Using a nitric chide (NO)-sensitive platinum microelectrode(SNM) modified withchitosan nickel (Ⅱ) complex and Nafion (Nation-CTS (Ni)-Pt), we observed the effectof nitric oxide synthase (NOS) inhibitor 7-nitro-indazole (7-NI) and N-nitro-L-arginine(L-NNA) on penicillin-treated hippocampal slices by simultaneously recording thestimulus-evoked field potentials and nitric oxide release from CA1 pyramidal neurons. Ourresults show: (1) The linear relationship range and detection limit of SNM were 4.5 ×10-4~ 1.0 ×10-8 mol/L and 5.0 × 10-8 mol/L respectively. (2) Penicillin (PEN)could elevate NO release, the number and amplitude of stimulus-evoked field potentialsspike (SEPS) in concentration-dependent manner; (3) Both 7-NI and L-NNA depressedNO release and partly reversed the effect of penicillin. The above findings suggest thatconvulsant provoking effect of NO could be inhibited by NOS inhibitors. The NO-sensitiveelectrode may provide an useful tool for continuous detection of NO in biological

关 键 词：癫痫 海马脑片 诱发痫波 一氧化氮 NO敏感电极 

分 类 号：Q424[生物学—神经生物学] R742.102[生物学—生理学]