Mapmarker/Exp3.0遗传图谱构建中重要参数设置初步探讨  

Selection Techniques of Some Important Parameters in Genetic Linkage Map Construction Using Mapmarker/Exp 3.0

作　　者：赖运平[1,2] 李俊[2] 董雪芳[1,2] 张泽全[1,2] 魏会廷[2] 彭正松[1] 胡晓蓉[2] 杨武云[2] 

机构地区：[1]西华师范大学生命科学学院,四川南充637002 [2]四川省农科院作物研究所,四川成都610066

出　　处：《西南农业学报》2009年第6期1508-1513,共6页Southwest China Journal of Agricultural Sciences

基　　金：国家自然科学基金(No.30771338和30871532);国家863计划(2006AA10ZIC6);国家小麦产业四川省国际合作项目;四川省应用基础研究;四川省育种工程和省育种攻关资助

摘　　要：Mapmarker/Exp(3.0)是目前分子作图中应用最广的软件之一,但由于许多重要参数的默认值并非是作图的最佳值,影响提高作图的精确性。本文以川麦42×川农16重组近交系(F8)作图群体为实例,对Mapmarker/Exp 3.0软件在遗传作图中不同LOD值、max distance值以及用compare and try、compare和order 3种排序方式对遗传图谱的影响进行了初步比较,结果表明,作图过程中,不应强求采取统一LOD值,对于特殊的连锁群可采取不同的LOD值;max distance值在20至50为宜,值过小容易遗漏大量本来正确连锁的标记;小于等于7个标记的连锁群可直接用compare命令排序。Mapmarker/Exp（3.0） is one of the most widely used software for linkage map constructing. However, the results of linkage map constructing would be affected by the optimum of the important and indispensable parameters. In present paper, the linkage maps using different LOD value, max distance and ordering commands were compared by using recombinant inbred lines of Chuanmai 42 and Chuannong 16. The result indicated that different LOD values should be used in different linkage groups, especially for some special linkage groups. The optimization value of max distance should range from 20 to 50, otherwise, many markers that original linkage together would be missing if the value is too low. The linkage group can directly use ＇ compare＇ command to get the marker sequence, when the used markers equal to or less than seven.

关 键 词：Mapmarker/Exp3.0 遗传连锁图谱 LOD值 

分 类 号：S03[农业科学]