肝刺激因子对肝癌细胞增殖的调节作用  

REGULATORY EFFECT OF HEPATIC STIMULATOR SUBSTANCE ON THE PROLIFERATION OF HUMAN HEPATOMA CELLS

作　　者：刘晓军[1] 安威[1] 雷天光 戎煜[1] 杜国光 

机构地区：[1]首都医科大学细胞生物系,北京100054

出　　处：《生理学报》1998年第5期543-550,共8页Acta Physiologica Sinica

基　　金：国家自然科学基金!No.39600053

摘　　要：初断乳雄性SD大鼠的肝匀浆以超速离心和柱层析法分离纯化肝刺激因子（HSS），观察其对肝癌细胞增殖、细胞表皮生长因子（EGF）受体表达及受体磷酸化的影响。结果表明，HSS具有明显的促肝癌细胞分裂增殖能力，提高细胞周期中S期细胞所占比例。HSS促肝癌细胞增殖作用与其促EGF受体表达有关，表现为：（1）HSS上调170kDEGF受体蛋白表达，此作用与EGF合用后明显加强，即是协同效应；（2）HSS上调EGF受体表达呈剂量-效应和时间-效应关系;（3）HSS可刺激EGF受体酪氨酸残基的磷酸化，调节受体活性。本研究结果提示，HSS可能是通过调节肝癌细胞EGF受体的表达及受体介导的信号传导通路而起到促增殖的作用。The hepatic stimulator substance (HSS) was prepared from weanling rat livers byultracentrifugation and Sephadex G-75 chromatography. The stimulatory effects of HSS oncell proliferation, EGF receptor expression and receptor tyrosine phosphorylation wereinvestigated in serum-free cultured human hepatoma cell line SMMC-7721. The resultsshowed: (1) The relative percentage of S-phase cells was increased in HSS-treated cells.(2) EGF receptor protein level was increased after the cells were beated with HSS for 12~24 h and this effect was dose- and time-dependent. (3) Treatment of the cells withHSS for 0. 5 ~ 15 min resulted in the autophosphorylation of EGF receptor tyrosine residuedramatically. The above results suggest that the proliferative action of HSS on hepatocytesfright be due to its modulation of EGF receptor expression and the signaling pathway.

关 键 词：肝刺激因子 表皮生长因子 肝细胞增殖 

分 类 号：R333.4[医药卫生—人体生理学] R735.7[医药卫生—基础医学]