小鼠性腺特异表达基因(GSE)的克隆及初步研究  

Molecular Cloning and Characterization of Mouse Gonad-specific Expression Gene(GSE)

作　　者：付强[1] 薛雯[1] 蒋宗良[1] 秦文松[1] 张明[1] 卢克焕[1] 

机构地区：[1]广西大学动物繁殖研究所,广西亚热带生物资源保护利用重点实验室,广西大学

出　　处：《西南农业学报》2009年第6期1776-1779,共4页Southwest China Journal of Agricultural Sciences

基　　金：广西自然科学基金项目(桂科自0640019)

摘　　要：从小鼠的睾丸中克隆了GSE(gonad-specific expression gene)基因,并对该基因进行序列分析和Southern、Northern杂交分析。核苷酸序列分析表明,GSE基因的ORF长度为745 bp,编码247个氨基酸,预测蛋白质分子量为27.6 kDa;Southern杂交证明GSE可能是单拷贝的基因;Northern杂交显示,GSE基因在小鼠体细胞组织(心脏、肝脏、肾脏、大脑、骨骼肌和脾脏)中没有检测到其表达,在睾丸中表达量很高,在卵巢中表达量较低。从推断出的氨基酸序列表明,GSE蛋白在细胞中可能是一个不带有信号肽的可溶性蛋白。In this study, a novel gene termed GSE （gonad-specific expression gene） was identified. Sequence analysis of GSE revealed that the open reading frame （ORF） was 745 bp, which encodes a protein of 247 aa with a predicted molecular mass of 27.6 kDa. Southern blot analysis revealed GSE gene was a single copy fragment. Northern blot analysis showed that this gene was abundantly expressed in mouse testis and slightly expressed in the mouse ovary. The deduced amino acid sequence indicated that GSE protein might be a soluble protein in the cytoplasm without signal peptide.

关 键 词：小鼠 GSE基因 GSE表达 SOUTHERN杂交 NORTHERN杂交 

分 类 号：S865.1[农业科学—野生动物驯养]