3-脱氧葡萄糖醛酮诱导新血管生成的实验研究  

Angiogenesis in Chorioallantoic Membrance Induced by 3-Deoxyglucosone

作　　者：江国荣[1] 张露蓉[1] 

机构地区：[1]南京中医药大学附属苏州市中医医院,江苏苏州215003

出　　处：《苏州大学学报（医学版）》2009年第5期858-861,共4页Suzhou University Journal of Medical Science

基　　金：江苏省中医药管理局资助项目(2003~2004);苏州市高层次人才资助项目(2004-33)

摘　　要：目的观察3-脱氧葡萄糖醛酮(3-DG)对新血管生长的影响。方法应用鸡胚绒毛尿囊膜(CAM)和内皮细胞体外培养的方法研究3-DG对新血管生长的影响。CAM血管生长采用单位面积血管百分比法评价;细胞增殖采用MTT法评价。结果0.1、1μg3-DG和0.1μgAGEs组均可促进CAM新生血管生成,与空白对照组比较,差异有统计学意义(P<0.05)。与内皮细胞孵育24h后,3-DG50、100、150、200和250mg/L浓度组均明显促进内皮细胞生长(P<0.05),而300、400mg/L浓度组则无促进作用(P>0.05);与内皮细胞孵育48h后,50、100、150和200mg/L浓度组均有促进内皮细胞生长的趋势,但差异均无统计学意义(P>0.05),250mg/L无作用呈下降趋势,而300和400mg/L浓度组则明显抑制内皮细胞生长(P<0.05)。形态学观察,3-DG300mg/L组与空白对照组比较,出现细胞核固缩。结论3-DG可以诱导新血管生长。Objective To observe the effect of 3-deoxyglucosone （3-DG） on angiogenesis. Methods （1） The effect of 3-DG on angiogenesis was examined in vivo by the improved chorioallantoic membrance（CAM） assay（including ＂the blood vessels percentage in certain area＂ ,a measure angiogenesis method on CAM）. （2） The effect of 3-DG on proliferation of vascular endothelial cells （ECV304） in vitro was assessed by MTT assay （490 nm）. Results （1） Compared with the controls,significantly angiogenesis on CAM was observed in the 3-DG （0.1,1 μg） and AGEs （0.1 μg） groups （P〈0.05）; （2） Incubated with ECV304 for 24 h,in the 3-DG （50,100,150,200 and 250 mg/L） groups the proliferation of ECV30 was able to be promoted （vs control,P〈0.05）,but no effect was observed in the 3-DG （300 and 400 mg/L） groups（vs control,P〉0.05）; Incubated with ECV304 for 48 h,in the 3-DG（300 and 400 mg/L） groups the proliferation of ECV304 was able to be inhibited （vs control,P〈0.05）,while no effect was observed in the 3-DG （50,100,150,200 and 250 mg/L） groups （vs control,P〉0.05）. （3） Morphologically shrink of the core was observed in the ECV304 which was incubated with 3-DG （300 mg/L） for 48h. Conclusion The results imply that 3-DG may be an angiogenetic factor which can induce abnormal angiogenesis.

关 键 词：3-脱氧葡萄糖醛酮 鸡胚绒毛尿囊膜 内皮细胞 新血管生成 

分 类 号：R587.202[医药卫生—内分泌]