胰腺B细胞显像剂^(99)Tc^m-DTPA-NGN2在小鼠体内的分布  

Biodistribution and Imaging of Functional Pancreatic B Cell Imaging Agent:^(99)Tc^m-DTPA-Nateglinide in Mice

作　　者：谢莹[1] 唐军[2] 刘志华[1] 杨仪[2] 

机构地区：[1]苏州大学附属第二医院内分泌科,江苏苏州215004 [2]苏州大学附属第二医院核医学科,江苏苏州215004

出　　处：《苏州大学学报（医学版）》2009年第5期924-926,共3页Suzhou University Journal of Medical Science

摘　　要：目的研究胰腺B细胞显像剂99Tcm-DTPA-NGN2在小鼠体内的分布规律,为99Tcm-DTPA-NGN2用于临床胰腺B细胞显像及胰腺癌的核医学显像做基础。方法正常小鼠42只,分为7组,每组6只,尾静脉注射99Tcm-DT-PA-NGN23.7MBq后5、15、30min和1、2、4、6h放血处死,取心、肝、脾、肺、脑、肾、肌肉、骨、小肠、胃、胰腺和血液等组织或器官,称重并测量其放射性,计算每克组织百分注射剂量率(%ID/g)。同时,取正常小鼠5只,尾静脉注射99Tcm-DTPA-NGN218.5MBq后相同时间进行显像。结果99Tcm-DTPA-NGN2主要经肾脏代谢,脑内未见摄取,各组织、器官的百分注射剂量率在1h内均迅速下降,胰腺的百分注射剂量率随时间的延长逐渐上升,在1～2h内保持较高水平。显像结果与生物分布实验结果一致,随时间的延长胰腺显影逐渐清晰,在1～2h内均可清晰观察胰腺组织。结论99Tcm-DTPA-NGN2可能是一种较好的胰腺B细胞显像剂,在胰腺B细胞的功能显像和胰腺癌显像的应用中值得进一步研究。Objective To evaluate the biodistribution and imaging of technetium -99m labeled Nateglinide：99^Tc^m-DTPA-Nateglinide （99^Tc^m-DTPA-NGN2） in mice. Methods Forty-two healthy Kun-ming mice were divided into 7 groups. Five minutes,15 minutes,30 minutes,1 hour,2 hours,4 hours,and 6 hours after the injection of 3.7 MBq 99^Tc^m-DTPA-NGN2,these mice were sacrificed by cutting the carotid. Selected tissues（heart,liver,spleen,lung,brain,kidney,muscle,bone,intestine,stomach,pan-creas and blood） were excised and weighed,and the radioactivity was measured. The biodistribution of radiotracer in each tissue sample was calculated as a percentage of the injected dose per gram of wet tissue weight （%ID/g）. Another 5 mice were imaged after the injection of 18.5 MBq 99^Tc^m-DTPA-NGN2 at the same time. Result 99^Tc^m-DTPA-NGN2 is metabolized mainly from kidney,brain doesn＇t uptake 99^Tc^m-DTPA-NGN2,the %ID/g of the selected tissues were decreased rapidly in 1 hour,and the radioactivity in pancreas kept stable from 1 hour to 2 hours after injection. The pancreas visualized clearly 1 hour after injection. Conclusion 99^Tc^m-DTPA-NGN2 can be clearly visualized in pancreas in mice,and maybe it＇s promising in imaging pancreatic tumor and in evaluating the function of pancreatic B cell.

关 键 词：胰腺B细胞 显像 99^Tc^m-DTPA-NGN2 小鼠 生物学分布 

分 类 号：R817.4[医药卫生—影像医学与核医学]