糖皮质激素对鼻息肉中固生蛋白C表达的抑制作用  

Inhibitory Effects of Glucocorticoids on the Tenascin C Expression in Nasal Polyp

作　　者：王恒[1] 刘争[1] 陆翔 游学俊[1] 高起学[1] 崔永华[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院耳鼻咽喉-头颈外科,武汉430030

出　　处：《华中科技大学学报（医学版）》2009年第6期756-759,共4页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

基　　金：国家自然科学基金(No.30500557);教育部留学回国人员科研启动基金(教外司留[2006]331号);教育部新世纪优秀人才支持计划(NCET-07-0326)资助项目

摘　　要：目的研究糖皮质激素对鼻息肉及呼吸道上皮细胞中固生蛋白C(tenascin C,TNC)表达的影响,探求糖皮质激素抑制鼻息肉组织结构重塑的可能机制。方法采用ELISA方法检测经鼻腔吸入糖皮质激素(布地奈德)治疗患者和未治疗患者的鼻息肉组织中TNC和转化生长因子β1(transforming growth factor-β1,TGF-β1)的蛋白含量;进一步采用细胞培养、实时定量RT-PCR和原位ELISA技术观察布地奈德对人呼吸道上皮细胞系BEAS-2B细胞TNC表达的调控作用。结果与未治疗组相比,治疗组鼻息肉组织中TNC和TGF-β1的蛋白表达明显减少(P<0.01);鼻息肉组织中TNC和TGF-β1的蛋白水平呈显著正相关(r=0.68,P<0.01)。经布地奈德预处理后,TGF-β1诱导的BEAS-2B细胞TNC mRNA和蛋白的表达明显减少(P<0.01)。结论糖皮质激素可以通过抑制鼻息肉组织中TGF-β1的表达及抑制TGF-β1上调呼吸道上皮细胞TNC表达的功能而参与对鼻息肉组织结构重塑的调控。Objective To investigate the effect of glucocorticoids on tenascin C（TNC）expression in nasal polyp tissues and airway epithelia,and explore the possible mechanism of glucocorticoids inhibiting remodeling of nasal polyp tissue.Methods The enzyme linked immunosorbent assay（ELISA）was used to detect the protein levels of tenascin C and transforming growth factor-β1（TGF-β1）in nasal polyp tissues from intranasal glucocorticoids（Budesonide,BUD）treated group and untreated group.The cell culture,real-time RT-PCR and in situ ELISA were employed to investigate the regulatory effects of budesonide on the TNC mRNA and protein expression in BEAS-2B immortalized human bronchial epithelial cells.Results The protein levels of TNC and TGF-β1 were decreased in nasal polyp tissues from intranasal BUD-treated group as compared with untreated group（P〈0.01）.There was a significantly positive correlation between TNC and TGF-β1 protein levels in nasal polyp tissues（r=0.68,P〈0.01）.After preincubation with BUD,TNC mRNA and protein expression induced by TGF-β1 in BEAS-2B cells was significantly decreased（P〈0.01）.Conclusion Glucocorticoids might participate in the regulation of tissue remodeling in nasal polyp by inhibiting the TGF-β1 expression in nasal polyp tissue and suppressing the induction of TGF-β1 to up-regulatie the TNC expression in airway epithelia.

关 键 词：糖皮质激素 鼻息肉 上皮细胞 固生蛋白C 转化生长因子Β1 

分 类 号：R765.25[医药卫生—耳鼻咽喉科]