应用激光扫描细胞仪分析食管癌中HP1-γ蛋白的表达  

The Analysis of the HP1-γ Expression in Different Grades of Esophageal Carcinoma by Using Laser Scanning Cytometer

作　　者：李伟[1] 董虹[1] 刘佳[1] 李双[1] 王薇[1] 马丁[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院妇科肿瘤实验室,武汉430030

出　　处：《华中科技大学学报（医学版）》2009年第6期764-767,共4页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

基　　金：国家"973"重大基础研究发展计划(No.2009CB521808);教育部新教师基金(No.200804871095);湖北省自然科学基金(No.2008CDB213)资助项目

摘　　要：目的了解异染色质相关蛋白1-γ(heterochro-Matin-associated protein 1,HP1-γ)在食管癌发生发展各阶段的表达情况,并初步探讨利用激光扫描细胞仪分析免疫组化结果较传统光镜下评分方法的优势。方法选用含各级别食管癌病变组织的组织芯片,采用免疫组化方法检测其中HP1-γ的表达,并通过激光扫描细胞仪对免疫组化结果进行定量分析。将激光扫描细胞仪分析结果和传统光镜下评分结果进行相关性分析。结果HP1-γ蛋白主要表达于胞核。在正常食管组织、中-重度异型增生、原位癌、食管鳞癌的阳性率分别为37.5%(3/8),100%(21/21)、100%(7/7)和23.7%(9/38),且在正常食管、中-重度异型增生加原位癌、食管鳞癌组织中的表达差异有统计学意义(P<0.01)。激光扫描细胞仪分析结果与传统光镜下评分结果具有高度相关性(P<0.01)。结论HP1-γ蛋白可能在食管正常细胞向恶性细胞转化的过程中起到了一定的抵抗作用。与传统人工评分方法相比,激光扫描细胞仪在分析免疫组化结果时具有高通量、高度客观、准确的优点。Objective To understand the heterochro-Matin-associated protein 1（HP1-γ）expression during the carcinogenesis and progression of esophageal carcinoma,and preliminarily investigate the superiority of using laser scanning cytometer to analyze immunohistochemistry results compared to traditional scoring methods.Methods A tissue microarray containing different grades of esophageal carcinoma was selected and the HP1-γ expression was detected by immunohistochemistry.The results of immunohistochemistry were analyzed quantitatively by using laser scanning cytometer.The correlation of results analyzed by using laser scanning cytometer and traditional scoring methods was analyzed by chi square test.Results The HP1-γ was primarily expressed in the nucleus.The positive rate of HP1-γ in normal esophagus,moderate-severe atypical hyperplasia,in situ carcinoma and squamous cancer was 37.5%（3/8）,100%（21/21）,100%（7/7）and 23.7%（9/38）,respectively,with the difference being statistically significant among n多ormal esophagus,moderate-severe atypical hyperplasia plus in situ carcinoma and squamous cancer（P〈0.01）.There was a high correlation between the results analyzed by laser scanning cytometer and those by traditional scoring methods under a light microscope（P〈0.01）.Conclusion HP1-γ may play a resistant role in the transformation from normal esophageal cells to malignant cells.Compared to the traditionally artificial scoring methods,there are many advantages such as high resolution,high objectivity and accuracy when using laser scanning cytometer to analyze the immunohistochemistry results.

关 键 词：异染色质相关蛋白1 食管癌 激光扫描细胞仪 组织芯片 

分 类 号：R392.11[医药卫生—免疫学]