I-1-P反义ODN-壳聚糖纳米粒子的制备及对结核杆菌生长的抑制作用  

Preparation of I-1-P Chitosan-antisense Nanoparticles and Their Inhibition against M.tuberculosis Growth

作　　者：沈杰[1] 陈志飞 张红菱[1] 李晓波[1] 卢实[3] 李媛媛[1] 

机构地区：[1]华中科技大学同济医学院公共卫生学院环境医学研究所,武汉430030 [2]武汉结核病医院,武汉430030 [3]华中科技大学同济医学院附属协和医院妇产科,武汉430022

出　　处：《华中科技大学学报（医学版）》2009年第6期771-774,共4页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

基　　金：国家自然科学基金资助项目(No.30170051;No.30328031)

摘　　要：目的探讨应用纳米技术抑制分枝结核杆菌生长的可行性。方法用复凝聚法制备I-1-P反义ODN-壳聚糖纳米粒子。在分枝结核杆菌菌株中分别加入不同量的自由ODN和壳聚糖-ODN纳米粒子,共同培养6周后观测细菌的生长情况。结果制得的纳米粒子由(35.6±0.9)%的ODN和(64.4±0.9)%的壳聚糖组成。壳聚糖-ODN纳米粒子浓度为4μmol/L时,其对分枝结核杆菌的抑制作用为(2.8±0.1)CFU/ml。而自由ODN在浓度低于10μmol/L时,不能有效抑制分枝结核杆菌的生长。结论壳聚糖-ODN纳米粒子比自由ODN对分枝结核杆菌的生长具有更强的抑制作用。Objective To investigate the feasibility of applying nanotechnology against M.tuberculosis growth.Methods Chitosan-antisense ODN nanoparticles were prepared by complex coacervation method.Varied amount of ODNs and chitosan-ODN nanoparticles were added to the cultures and the growth of the bacilli was monitored.Results The nanoparticles were composed of（35.6±0.9）% ODN and（64.4±0.9）% chitosan.Compared to the free ODN,antisense nanopartilces were more effective in inhibiting the proliferation of M.tuberculosis.Antisense nanoparticles decreased growth by（2.8±0.1）CFU/ml at concentration of 4 μmol/L.Conclusion Chitosan-ODN nanoparticles were more effective in inhibiting the growth of M.tuberculosis than free ODN.

关 键 词：壳聚糖 肌醇-1-磷酸合成酶反义硫代寡核苷酸 复合纳米粒子 结核杆菌 

分 类 号：R378.911[医药卫生—病原生物学]