RECK基因在子痫前期患者胎盘的表达及其与MMP-2活化的关系  

Expression of RECK Gene in Placentas from Patients with Preeclampsia and Its Correlation with MMP-2 Activation

作　　者：郭君红[1] 徐亮[2] 张文淼[1] 陈云琴[1] 周静[1] 程晓红[1] 黄引平[1] 

机构地区：[1]温州医学院附属第一医院妇产科,温州325000 [2]三峡大学人民医院骨科,宜昌443000

出　　处：《华中科技大学学报（医学版）》2009年第6期818-820,831,共4页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

摘　　要：目的通过检测RECK基因在子痫前期患者胎盘组织中的表达及其与MMP-2活化的关系,探讨RECK基因在胎盘滋养细胞浸润机制中的作用。方法分别采用RT-PCR、Western blot检测正常妊娠孕妇(正常妊娠组,22例)、子痫前期孕妇(其中轻度22例,重度20例)胎盘组织中RECK mRNA和蛋白的表达;采用明胶酶谱法检测3组孕妇胎盘组织中MMP-2的活化比例。结果轻度、重度子痫前期组胎盘组织中RECK mRNA、蛋白的表达水平均明显高于正常妊娠组,且重度子痫前期组RECK mRNA、蛋白的表达水平均明显高于轻度子痫前期组,组间比较差异均有统计学意义(均P<0.01);而轻度、重度子痫前期组MMP-2的活化比例均明显低于正常妊娠组,且重度子痫前期组MMP-2的活化比例明显低于轻度子痫前期组,组间比较差异均有统计学意义(均P<0.01)。正常妊娠组、轻度子痫前期组、重度子痫前期组胎盘组织中RECK mRNA及其蛋白表达与MMP-2的活化比例均呈负相关关系(均P<0.01)。结论子痫前期孕妇胎盘组织中RECK表达异常升高,MMP-2活性受到抑制,可能参与了胎盘滋养细胞浅浸润过程。Objective To investigate the expression of RECK gene in placentas of patients with preeclampsia and its correlation with MMP-2 activation,and explore the possible roles of RECK gene in the placental trophoblast invasion mechanism.Methods RT-PCR and Western blot were used to detect the expression of RECK mRNA and protein respectively in the placental tissues of normal late pregnant women（normal pregnant group,22 cases）and pre-eclamptic patients（22 mild cases and 20 severe cases）.Gelatinase zymography was used to determine MMP-2 activation ratio.Results The expression levels of RECK mRNA and protein from placenta tissues in mild,severe pre-eclamptic group were both significantly higher than those in normal pregnant group.Moreover,the expression levels of RECK mRNA and protein in severe pre-eclamptic group were obviously increased as compared with those in mild pre-eclamptic group.There was significant difference among the three groups（all P〈0.01）.MMP-2 activation ratio in mild,severe 多pre-eclamptic group was significantly lower than that in normal pregnant group.MMP-2 activation ratio in severe pre-eclamptic groups was obviously reduced as compared with mild pre-eclamptic group.There was significant difference among the three groups（all P〈0.01）.The expression levels of RECK mRNA and protein were significantly negatively correlated with MMP-2 activation ratio（both P〈0.01）.Conclusion The abnormal high expression of RECK and inhibition of MMP-2 activation in placentas of pre-eclamptic patients may participate in the process of placental trophoblast shallow invasion.

关 键 词：RECK基因 胎盘 子痫前期 基质金属蛋白酶 

分 类 号：R714.245[医药卫生—妇产科学]