作用于HBV(adr亚型)RNA的tRNA-包埋锤头状核酶的研究  

tRNA embedded Hammerhead Ribozymes Mediate Destruction of HBV(Subtype adr) in vitro

作　　者：郑文超[1] 祁国荣[1] 

机构地区：[1]中国科学院上海生物化学研究所

出　　处：《生物化学与生物物理学报》1998年第5期432-438,共7页

基　　金：国家高技术"863"计划

摘　　要：设计了针对ＨＢＶ（ａｄｒ亚型）ＲＮＡ的二个锤头状核酶（ＲＳ３和ＲＣ２），并将其插入ｔＲＮＡ反密码环中（ＲｔＳ３和ＲｔＣ２），以增加其稳定性。实验表明，虽然插入ｔＲＮＡ中的核酶与裸露核酶相比，催化活性有所下降，但在胎牛血清和ＨｅｐＧ２细胞抽提液中的稳定性却明显提高。因此，ｔＲＮＡ－包埋核酶有可能提高在体内的抗病毒能力。Two hammerhead ribozymes, RS3 and RC2, targeting the HBsAg and HBcAg regions of HBV( adr) RNA respectively, have been designed. To improve the stability of ribozymes, two tRNA embedded ribozymes, RtS3 and RtC2, were obtained by inserting RS3 and RC2 into the anticodon region of tRNA. Although the catalytic activity of the tRNA embedded ribozymes was lower than that of their naked counterparts, the stability of the former in fetal bovine serum or cytoplasmic extract of HepG2 cells was much higher than that of the naked ribozymes. These results suggest that the antivirus effect of tRNA embedded ribozymes may be greater in vivo in comparison with naked ribozymes.

关 键 词：锤头状核酶 HBV 自剪切质粒 tRNA-包埋 

分 类 号：Q55[生物学—生物化学]