17个新的C2H2型锌指基因片段的分离与克隆  

Isolation and Cloning of 17 Novel C 2H 2 Zinc Finger Gene Fragments

作　　者：张民[1] 余龙[1] 胡培蓉[1] 王小柯[1] 毕安定[1] 陈琪[1] 赵寿元[1] 

机构地区：[1]复旦大学遗传学研究所遗传工程国家重点实验室

出　　处：《生物化学与生物物理学报》1998年第5期465-470,共6页

基　　金：国家863高技术项目;国家杰出青年科学基金;国家自然科学基金

摘　　要：按照Ｃ２Ｈ２型锌指基因保守结构域的ＤＮＡ序列设计一对简并引物，以人基因组ＤＮＡ为模板进行ＰＣＲ同源扩增，将由此获得的锌指基因片段为探针，从人胎肾、骨骼肌、骨髓组织的ｃＤＮＡ分子库中筛选到２２个Ｃ２Ｈ２型锌指蛋白ｃＤＮＡ片段，经国际ＮＣＢＩ数据库查询检索，其中１７个为新的锌指基因片段。对从胎肾ｃＤＮＡ分子库中分离到的Ｋ３－４和Ｋ５－１２克隆进行了表达谱分析，发现Ｋ３－４在肾脏中的表达量明显高于其他几种组织，Ｋ５－１２则在８种组织中均有不同程度的表达。A pair of degenerate primers were designed according to the DNA sequences of the C 2H 2 zinc finger genes conservative domain and then homologious PCR was performed with the human genomic total DNA as template. The zinc finger fragments thus obtained were used as probes to screen cDNA libraries of human fetal kidney, muscle and marrow and 22 C 2H 2 zinc finger gene fragments were selected. Among these fragments, 17 were confirmed to be novel zinc finger gene fragments by literature searches in the National Center Biotechnology Information (NCBI) database. Expression patterns of the clones K3 4 and K5 12 selected from kidney cDNA library were analyzed . The results showed that the expression level of K3 4 in kidney is obviously higher than in other tissues and K5 12 is expressed at different levels in 8 tissues.

关 键 词：C2H2型锌指基因 同源扩增 表达谱分析 克隆 

分 类 号：Q78[生物学—分子生物学]