玉米叶绿体ATP合成酶ε亚基的定点突变  

Site directed Mutagenesis of ε Subunit of ATP Synthase of Maize Chloroplast

作　　者：史瑾[1] 魏家绵[1] 沈允钢[1] 

机构地区：[1]中国科学院上海植物生理研究所

出　　处：《生物化学与生物物理学报》1998年第5期483-487,共5页

基　　金：国家自然科学基金;植物分子遗传国家重点实验室基金

摘　　要：利用突变引物和ＰＣＲ方法对玉米叶绿体ＡＴＰ合成酶的ε亚基进行定点突变，使ε亚基４２位上的Ｔｈｒ分别替换成Ｃｙｓ、Ａｒｇ、Ｉｌｅ和Ｐｒｏ。Ｔｈｒ变为Ｐｒｏ的ε亚基突变体不能表达，其他突变体与野生型一样，都能正常表达。Ｔｈｒ突变为Ｃｙｓ和Ａｒｇ后，突变体对ＡＴＰａｓｅ活力的抑制比野生型略高一些，但突变为Ｉｌｅ后，其抑制程度则极大地增强了。The effects of some residues in maize chloroplast ε subunit on its activity have been studied by site directed mutagenesis. After replacing Thr 42 of ε subunit with Cys, Arg, Ile or Pro, and forming the mutant εT42C, mutant εT42R, mutant εT42I and mutant εT42P respectively, it was found that the mutant εT42P protein could no longer be expressed, but expression of other ε subunit mutants was similar to that of wild type. Comparing the inhibitory potency of different mutants of ε subunit with that of the wild type, it was found that the inhibitory effects of ε subunit mutants εT42C and εT42R on ATPase activity were slightly higher than that of wild type, but the εT42I protein strongly inhibited the Ca 2+ ATPase activity.

关 键 词：定点突变 Ε亚基 ATPase活力 玉米 

分 类 号：Q550.1[生物学—生物化学] S513.032[农业科学—作物学]