神经肽AVP(4-8)激活大鼠海马突触膜上的G蛋白偶联受体  

AVP(4 8) May Stimulate a G Protein coupled Receptor in Rat Hippocampal Synaptosomal Membranes

作　　者：阎庆武[1] 杜雨苍[1] 

机构地区：[1]中国科学院上海生物化学研究所

出　　处：《生物化学与生物物理学报》1998年第5期505-509,共5页

基　　金：国家攀登计划;国家自然科学基金;上海生命科学研究中心资助

摘　　要：精氨酸加压素（ＡＶＰ）的Ｃ端内片段，ＡＶＰ（４－８），具有增强记忆的功能，它在大鼠脑内引发一系列的生理和生化变化。ＧＴＰ－结合调节蛋白（Ｇ蛋白）介导多数神经肽和神经调质的细胞内生理生化反应。放射性受体结合实验显示，在海马突触膜上存在ＡＶＰ（４－８）的特异性结合位点。ＡＶＰ（４－８）在海马突触膜上的结合能够进一步刺激ＧＴＰγＳ结合并可被ＡＶＰ（４－８）的受体拮抗剂ＺＤＣ（Ｃ）ＰＲ所逆转。从以上实验结果可以认为ＡＶＰ（４－８）是通过海马突触膜上的Ｇ蛋白偶联受体来发挥其神经调质作用的。As a metabolite of arginine vasopressin, AVP(4 8) has been shown to have potent memory enhancing activity and to induce a series of physiological and biochemical events in rat brain. GTP binding protein is known to be a revolving stage of transmembrane signal transduction to mediate physiochemical responses of neurotransmitters and neuromodulators. A specific binding site of AVP(4 8) in the rat hippocampal synaptic membranes was identified by radio receptor assay and after binding to membranes, AVP(4 8) enhanced the binding of Guanosine 5′ O (3 [ 35 S]thio)triphosphate ([ 35 S]GTPγS), and this enhancement could be completely reversed by the antagonist of AVP(4 8), ZNC(C )PR. Based on the alone results, we suggest that AVP(4 8) exerts its function as neurotransmitter through a G protein coupled receptor on the synaptosomal membrane of rat hippocampus.

关 键 词：AVP(4-8) 受体结合 GTPγS G蛋白偶联受体 海马 

分 类 号：Q51[生物学—生物化学]