彗星电泳法在植物原生质体凋亡检测中的应用  

APPLICATION OF COMET ASSAY IN PLANT PROTOPLAST APOPTOSIS DETECTION

作　　者：姜晓芳[1] 朱海珍[1] 周军[1] 陈浩明[1] 戴尧仁[1] 

机构地区：[1]清华大学生物科学与技术系

出　　处：《Acta Botanica Sinica》1998年第10期928-932,共5页Acta Botanica Sinica（植物学报：英文版）

摘　　要：应用中性法彗星电泳检测烟草（ＮｉｃｏｔｉａｎａｔａｂａｃｕｍＬ．）原生质体的凋亡。结果表明，彗星发生的百分率（彗星率）和发生核质固缩及“核着边”（凋亡形态学标志）的细胞的百分率之间存在明确的相关性。利用标准的细胞凋亡检测手段，包括ＤＮＡｌａｄｄｅｒｉｎｇ及核糖核酸末端转移酶介导的ｂｉｏｔｉｎｄＵＴＰ缺口末端标记（ＴＵＮＥＬ），都证明这种彗星电泳法可以用来比较精确地检测植物原生质体的凋亡。The authors report the application of neutral comet assay in the detection of apoptosis in tobacco (Nicotiana tabacum L.) protoplasts. The results suggested a close inter relationship between comet formation and nuclear compacting into densed masses at the nuclear periphery (a typical morphological symptom of apoptosis). Standard detection of hallmarks of apoptosis, including DNA laddering and TdT mediated biotin dUTP nick end Lase Labeling (TUNEL), was also performed in order to conform the reliability of comet assay in the detection of apoptosis in plant protoplasts.

关 键 词：中性法彗星电泳 细胞凋亡 DNA断裂 烟草原生质体 

分 类 号：Q942.5[生物学—植物学]