玉米反式因子基因分离初报  

PRELIMINARY REPORT ON ISOLATION OF A TRANS ACTING FACTOR GENE FROM ZEA MAYS 

作　　者：萧灿[1] 金振华[1] 马诚[1] 

机构地区：[1]中国科学院发育生物学研究所

出　　处：《Acta Botanica Sinica》1998年第10期977-980,共4页Acta Botanica Sinica（植物学报：英文版）

基　　金：国家自然科学基金

摘　　要：发育是一个严格有序的选择性基因表达过程。在这一过程中，序列特异的反式因子（ｔｒａｎｓａｃｔｉｎｇｆａｃｔｏｒｓ）通过与基因调控序列中顺式调控元件（ｃｉｓａｃｔｉｎｇｅｌｅｍｅｎｔｓ）的互作启动基因转录，因此，它们又被称为转录因子［１］。基于这类因...Eight clones were isolated from maize (Zea mays L.) λgt11 cDNA expression library using 5′ upstream regulatory sequence of DC59 as the probe and screened from carrot somatic embryo cultures. Interaction assays were performed using the systematic deletion fragments of 5′ upstream regulatory sequence and the expressed proteins of each clone and gel retardation assay and the competitive binding tests were carried out. The results indicated that protein G10 expressed from the corresponding clone G10 was able to bind at -295 to -221 bp of the regulatory sequence specifically. This region contains a pair of direct and inverted repeats GGAA and GGAA as well as GGAA and TTCC,respectively, and a putative plant hormone abscisic acid responsive element ACGTGG. Predicted secondary structure demonstrated that protein G10 encompassed DNA binding domains. This is further borne out by sequence homology comparison with other known transcription factors. So the clone G10 possesses several properties as a new trans acting factor gene related to embryo specific genes.

关 键 词：DC59.5′上游调控序列 玉米cDNA表达文库 反式因子 

分 类 号：S513.032[农业科学—作物学]