RICE TRANSFORMATION WITH A PHYTOALEXIN GENE AND BIOASSAY OF THE TRANSGENIC PLANTS   

植物抗毒素转化水稻和转基因植株的生物鉴定(英文)

作　　者：田文忠[1] 丁力[1] 曹守云[1] 戴顺洪[1] 叶松青[1] 李良材[1] 

机构地区：[1]中国科学院遗传研究所

出　　处：《Acta Botanica Sinica》1998年第9期803-808,共6页Acta Botanica Sinica（植物学报：英文版）

摘　　要：Immature embryos, mature embryos and embryogenic calli of 6 rice (Oryza sativa L.) materials were transformed with particle bombardment. The plasmids pSSVstl and pVE5+ were used, both containing the phytoalexin gene from grapevine coding for stilbene synthase, but driven by 35S and its own promoter respectively. Through resistance selection for G418 (100 to 150 mg/L) or hygromycin (50 mg/L), 54 independent transgenic plants were isolated and further assessed by PCR, Southern blot and Dot blot analyses. The transgenic plants and their progenies were tested for resistance to blast ( Pyricularia oryzae ) and bacterial blight of rice ( Xanthomonas oryzae ). Preliminary results indicated that the stilbene synthase gene could enhance the resistance of transgenic plants and their progenies to both pathogens.用基因枪法转化了水稻（ＯｒｙｚａｓａｔｉｖａＬ．）６个材料的未成熟胚、成熟胚及胚性愈伤组织。质粒ｐＳＳＶｓｔ１和ｐＶＥ５＋是由葡萄中分离出的编码芪类合成酶的植物抗毒素基因与３５Ｓ或它自己的启动子组成。Ｇ４１８（１００～１５０ｍｇ／Ｌ）或潮霉素（５０ｍｇ／Ｌ）筛选后，经ＰＣＲ、Ｓｏｕｔｈｅｒｎｂｌｏｔ或Ｄｏｔｂｌｏｔ分析证明的转基因植株共５４株。对转基因植株及其后代进行了稻瘟病和白叶枯病的抗性鉴定。初步结果表明，芪类合成酶基因可以提高转基因植株及后代的抗性。

关 键 词：Rice transformation PHYTOALEXIN Stilbene synthase Resistance to blast and bacterial blight of rice 

分 类 号：Q943[生物学—植物学] S511.035[农业科学—作物学]