用470fs时间分辨率荧光光谱法研究光系统Ⅱ反应中心原初反应的动力学特性  

STUDIES ON THE KINETIC PROPERTIES OF PHOTOSYSTEM Ⅱ PRIMARY REACTION USING FLUORESCENCE SPEC TROSCOPY WITH 470 FS TIME RESOLUTION 

作　　者：侯建敏[1,2] 匡廷云[1,2] 陈耀东 叶彤[1,2] 杨昆云 崔岩[1,2] 唐崇钦[1,2] 王水才 侯洵 汤佩松[1,2] 

机构地区：[1]中国科学院植物研究所光合作用基础研究开放实验室 [2]中国科学院西安光学精密机械研究所瞬态光学技术国家重点实验室

出　　处：《Acta Botanica Sinica》1998年第8期715-720,共6页Acta Botanica Sinica（植物学报：英文版）

基　　金：国家"攀登计划"重大基础项目;国家自然科学基金;中国科学院院长基金

摘　　要：以菠菜（ＳｐｉｎａｃｉａｏｌｅｒａｃｅａＭｉｌ．）叶绿体中的ＰＳⅡ颗粒和ＰＳⅡ核心复合物为材料，用４７０ｆｓ时间分辨率的荧光光谱技术研究ＰＳⅡ反应中心原初反应的动力学特性，选择不同的时间测量范围和不同的检测波长，经过解卷积和多指数拟合可以分辨出２～４个衰减组分，对所得的动力学参数进行分析和讨论，认为其中３ｐｓ的组分与电荷分离有关，而０．８、１２、２５和１００ｐｓ的衰减组分很可能属于能量传递过程，提出了可能的动力学模型。The primary reaction kinetics of the isolated photosystem Ⅱ particles and photosystem Ⅱ core complexes from spinach (Spinacia deracea Mill.) was investigated using the time resolved fluorescence spectroscopy with 470 fs time resolution. 2 to 4 lifetime components were detected by the multi exponential curve fitting method. These components were analyzed and discussed in terms of different kinetic processes. It is suggested that 3 ps component is attributed to the charge separation and 0.8 ps, 12 ps, 25 ps and 100 ps components are related to the energy transfer processes. A possible kinetic scheme in photosystem Ⅱ reaction center was proposed based upon the reported previously result.

关 键 词：光合作用 原初反应 光系统II 动力学 

分 类 号：Q945.11[生物学—植物学]