樱桃根癌土壤杆菌及其对土壤杆菌素84敏感性的研究  

ISOLATION AND IDENTIFICATION OF AGROBACTERIUM SPP. FROM CHERRY CROWN GALLS AND THEIR SENSITIVITIES TO AGROCIN 84

作　　者：王慧敏[1] 隋新华 李健强[1] 戴秀玉[2] 马德钦[2] 王建辉 刘继世 

机构地区：[1]中国农业大学植物病理学系,北京100094 [2]中国科学院微生物研究所,北京100080

出　　处：《微生物学报》1998年第5期381-385,共5页Acta Microbiologica Sinica

基　　金：北京市自然科学基金

摘　　要：从山东、河北、辽宁等地樱桃园的樱桃冠瘿瘤和土壤样品中分离到46株根瘤土壤杆菌。经鉴定有4株是Agrobacteriumtumefaciens（原生物型1），其余42株是A.rhizogenes（原生物型2）。这些菌株所诱导的冠瘿瘤中均合成胭脂碱（nopaline），属胭脂碱型Ti质粒的根癌土壤杆菌，并对放射土壤杆菌K84菌株所产生的土壤杆菌素84敏感。由于K84菌株对含胭脂碱Ti质粒的根癌土壤杆菌有很好的抑制效果，因此，用K84菌株防治樱桃根癌病是有应用前景的．Crown galls were sampled from cherry yards of Shandong, Hebei, and Liaoning Provinces. 46 pathogenic strains were isolated. Physiological and biochemical tests revealed that 4 stains were Agrobacterium tumefaciens(bio. l) and that the other 42 strains were A. rhizogenes (bio. 2), according tO the classifications and nomenclatUres of the genus Agrobacterium and the species revised by Sawada et al and Bouzar. The Ti plasheds of all strains were 'opaline type. All strains were sensihve to agrocin 84, which suggested that crown gall disease of cheap could be controlled by K84 sabin.

关 键 词：土壤杆菌 樱桃 根癌病 TI质粒 土壤杆菌素84 

分 类 号：S154.3[农业科学—土壤学] S432.42[农业科学—农业基础科学]