三平正并殖吸虫发育过程中的同工酶谱  

ISOZYME PATTERNS DURING DEVELOPMENT OF EUPARAGONIMUS CENOCOPIOSUS

作　　者：吴信忠[1] 李树华[1] 童新华[1] 

机构地区：[1]重庆医科大学寄生虫学教研室

出　　处：《动物学报》1998年第3期286-292,共7页ACTA ZOOLOGICA SINICA

基　　金：国家自然科学基金

摘　　要：采用同工酶电泳技术对三平正并殖吸虫童虫、早期成虫和后期成虫的葡萄糖磷酸变位酶、葡萄糖磷酸异构酶和葡萄糖６磷酸脱氢酶的同工酶进行了研究，结果显示三平正并殖吸虫由童虫向成熟期发育过程中，葡萄糖磷酸变位酶和葡萄糖磷酸异构酶的同工酶谱趋于复杂，酶带数目后期成虫＞早期成虫＞童虫；而葡萄糖６磷酸脱氢酶同工酶谱则趋于简单，童虫或未成熟虫体酶带数目较成熟虫体为多。三种酶系统的同工酶排列型式显示，吸虫由童虫向后期成虫发育过程中，其早期成虫呈现“过渡”型酶谱特征，但童虫期酶谱与早期成虫酶谱相似性较大。Studies on the isozymology of Euparagonimus cenocopiosus Chen during development were carried out with vertical PAGE. The enzyme electropherograms of phosphoglucomutase (PGM,E.C.2.7.5.1), glucosephosphate isomerase (GPI,E.C.5.3.1.9) and glucose 6 phosphate dehydrogenase (G6PD,E.C.1.1.1.49) in the juvenile, early adult and post adult of Euparagonimus cenocopiosus were compared. 2 Electrophoretic analyses revealed obviously that there were qualitative and quantitative variations in the isozyme patterns from the juvenile to post adult. The complexity of electropherogram patterns of PGM and GPI was increasing with age while that of G6PD was decreasing. 3 The isozyme patterns of early adult were intermediate forms between those of the juvenile and post adult, but more similar to those of the juvenile.

关 键 词：并殖吸虫 三平正并殖吸虫 同工酶 个体发育 

分 类 号：Q959.152[生物学—动物学] Q55