脱氢表雄酮对人早期胎盘孕酮分泌的影响  

EFFECT OF DEHYDROEPIANDROSTERONE ON PROGESTERONE SECRETION BY HUMAN EARLY PLACENTA IN VITRO

作　　者：周祖平[1] 郑祥建[1] 焦丽红[1] 张崇理[1] 

机构地区：[1]中国科学院动物研究所计划生育生殖生物学国家重点实验室

出　　处：《动物学报》1998年第3期341-346,共6页ACTA ZOOLOGICA SINICA

摘　　要：采用绒毛组织体外孵育模型，观察了脱氢表雄酮对人早期胎盘滋养层组织孕酮分泌的可能影响。结果表明，高浓度（１０－６ｍｏｌ／Ｌ以上）的脱氢表雄酮及其硫酸盐可以显著地抑制体外孵育的人早期胎盘滋养层组织的孕酮分泌（Ｐ＜０００１），低浓度则无效应。脱氢表雄酮及其硫酸盐抑制孕酮分泌的影响不依赖于其芳香化产物雌激素。芳香化酶抑制剂４羟基雄烯二酮也能抑制孕酮分泌（Ｐ＜０００１），但其作用可被１７β－雌二醇逆转。推测脱氢表雄酮及其硫酸盐抑制滋养层组织孕酮分泌的效应可能是其自身的功能，并依赖于其含量水平。The effect of dehydroepiandrosterone(DHEA)on progesterone production in vitro by huamn trophoblast at 6～8 weeks has been investigated.The tissue explants(1mm 3), placed in medium 199 containing either no steroid or various amounts of DHEA,DHEAS and/or 10 -5 mol/L of other chemicals, were incubated for 16 hours in air at 37℃ with vibration. The medium was then collected and analyzed for progesterone by RIA. Higher concentrations(≥10 -6 mol/L)of DHEA and DHEAS markedly suppressed progesterone secretion( P <0 001). The inhibitory action of DHEA(s) was not dependent on aromatization to estrogen. Significant reduction in progesterone formation was also caused by the aromatase inhibitor 4 hydroxyandrosterone( P <0 001). The action of the aromatase inhibitor could be reversed by concomitant addition of 17β estradiol, however, 17β estradiol alone had no effect. The present results suggest that the inhibitory effect of DHEA might result from its direct action on trophoblast cells and mainly depends on its own concentration.

关 键 词：脱氢表雄酮 人 早期滋养层组织 孕酮 

分 类 号：Q954.4[生物学—动物学]