用RAPD标记研究对虾属六个种间的亲缘关系  

STUDIES ON GENETIC VARIATION AND PHYLOGENETIC RELATIONSHIPS AMONG SIX SPECIES OF PENAEUS GENUS BY RAPD MARKERS

作　　者：宋林生[1] 相建海[1] 李晨曦 周令华[1] 刘瑞玉[1] 樊拥军[2] 

机构地区：[1]中国科学院海洋研究所 [2]南开大学生物学系

出　　处：《动物学报》1998年第3期353-359,共7页ACTA ZOOLOGICA SINICA

基　　金：国家科委攀登计划B项目;国家自然科学基金;山东科委重点基金项目;中国科学院院长基金支持项目;国家博士后基金课题中博基

摘　　要：用ＲＡＰＤ技术对对虾属中六种对虾基因组ＤＮＡ的多态性进行研究。在事先优化的反应条件下用２０个随机引物扩增，共得到３６４条清晰稳定的多态性片段，片段长度在２３０～２８００ｂｐ之间。统计这些片段，根据扩增片段的共享度计算出相对遗传距离指数，然后用ＵＰＧＭＡ和ＮＪ等聚类方法对其进行分析，构建系统树，确定了它们相互间的亲缘关系。从聚类分析的结果可以看到，外部形态比较相似的种类在基因组ＤＮＡ上表现出较大的相似性，反之则较小。Random amplified polymorphic DNA (RAPD) technique was applied to assess the genetic variations among six species in the genus Penaeus. Amplifications with 20 primers under predetermined optimal reaction conditions gave 364 reproducible fragments ranging from 230 to 2 800bp. The amplified products were scored as present (1) or absent (0) for each DNA sample and an index of genetic similarity (F) was calculated. The value of (1 F) was used to evaluate genetic distances between species and a phylogenetic tree was constructed. From these results, we find that the species with similar morphological characters share a higher similarity on genomic DNA.Otherwise, the similarity is lower.

关 键 词：RAPD 对虾属 亲缘关系 种间关系 

分 类 号：Q959.223.5[生物学—动物学]