藏獒基因组DNA提取方法的比较研究  

Comparison of the Genome DNA Extraction Methods in Tibetan Mastiff

作　　者：兰小平[1] 李一婧[1] 马纲[1] 万红玲[1] 张敏[1] 张海林[1] 

机构地区：[1]天水师范学院生命科学与化学学院,甘肃天水741001

出　　处：《天水师范学院学报》2009年第5期7-9,共3页Journal of Tianshui Normal University

摘　　要：分别采用酚-氯仿法和改进的盐析法对藏獒血液基因组DNA进行了比较提取,并以提取的基因组DNA为模板进行了RAPD-PCR扩增.结果表明,酚-氯仿法提取的藏獒血液基因组DNA在浓度和纯度上均显著的高于改进的盐析法(P<0.05),但两种方法所提取的藏獒基因组DNA都可用于RAPD分析.The methods of phenol-chloroform and improved salting out were used for whole blood genomic DNA Extraction of Tibetan Mastiff, and the RAPD fragments amplified with DNA template extracted by two methods.The results showed that the concentration and purity of genome DNA by the method of phenol-chloroform were significantly higher than the method of improved salting out （P〈0.05）,but extracted genome DNA by the two methods could be used for RAPD analysis of Tibetan Mastiff.

关 键 词：藏獒 基因组DNA 提取方法 RAPD 

分 类 号：Q78[生物学—分子生物学]