1996年延边地区无菌性脑膜脑炎病因病毒的分离及初步鉴定  

AN ETIOLOGICAL STUDY OF MENINGOENCEPHALITIS WHICH BROKE OUT IN YANBIAN PREFECTURE IN 1996

作　　者：陈立[1,2,3] 李玉雨[1,2,3] 郑红[1,2,3] 李主宣 李杰 蒋小泓[1,2,3] 尹永日[1,2,3] 李英信 方勇 祝双利 张礼璧[1,2,3] 

机构地区：[1]中国预防医学科学院病毒学研究所 [2]延边大学医学院 [3]延边医院

出　　处：《病毒学报》1998年第3期229-233,共5页Chinese Journal of Virology

摘　　要：吉林省延边地区１９９６年６月发生无菌性脑膜脑炎流行，在全地区２１６万人口中，发病人数约５～６千人，死亡２人，为历史上所罕见。从病人脑脊液和粪便标本中分离到多株病毒，分离率较高（分别达到５２．４％和６６．７％）。用ＷＨＯ提供的ＲＩＶＭ肠道病毒组合血清进行中和定型，不能确定型别。ＲＴ－ＰＣＲ结果表明为肠道病毒。病人早期血清特异性ＩｇＭ抗体阳性率７２．０％，证明所分离的病毒为此次无菌性脑膜脑炎暴发流行的病因。此病毒为肠道病毒，有可能是肠道病毒的一个新型别或变异株。An aseptic meningoencephalitis broke out in Yanbian prefecture of Jilin Province in June,1996.Among 2.16 millions population of Yanbian prefecture,there were about 5 000 patients, and two of the patients died. A total of 23 virus strains were isolated from the cerebrospinal fluid (CSF) and stool specimens of the patients using RD cell, the isolation rate of CSF was 52.4%,that of stool specimens was 68.4%.The isolated viruses could not be typed by neutralization test using the RIVM antiserum provided by WHO.RT-PCR showed the viruses were enteroviruses.The positive rate of patients' acute stage serum IgM antiboudy against the isolated virus was 72.0% by ELISA.The results demonstrate the isolated virus is the pathogen of the aseptic meningoencephalitis, it is an enterovirus, and it may be a new type or a mutated enterovirus strain.The further study about this virus is going on.

关 键 词：无菌性脑膜脑炎 肠道病毒 RT-PCR 分离 鉴定 

分 类 号：R373.31[医药卫生—病原生物学]